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WBBiB

Wydział Biochemii, Biofizyki i Biotechnologii

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Department of Microbiology

http://micro.wbbib.uj.edu.pl/

Prof. dr hab. Jan Potempa
room: B220 (3.1.24), phone: +48 12 664 63 43, e-mail: jan.potempa@uj.edu.pl

Prof. dr hab. Jacek Międzobrodzki, Professor
room: C242 (2.1.32), phone: 12 664 63 71, mail: jacek.miedzobrodzki@uj.edu.pl

Prof. dr hab. lek. med. Piotr Mydel, Professor
room: C225 (2.1.29), phone: 12 664 61 10, mail: piotr.mydel@uj.edu.pl

Dr hab. Joanna Kozieł, Associate Professor
room: B204 (3.1.36), phone: 12 664 63 62, mail: joanna.koziel@uj.edu.pl 

Dr hab. Maja Kosecka-Strojek, Assistant Professor
room: B205 (3.1.35), phone: 12 664 63 65, mail:maja.kosecka-strojek@uj.edu.pl

Dr Karina Adamowicz, Assistant Professor
room: B219 (3.1.23), phone: 12 664 63 77, mail: karina.adamowicz@uj.edu.pl

Dr Amir Aliramezani, Assistant Professor
pokój: C243 (2.1.31), telefon: 12 664 61 29, mail: amir.aliramezani@uj.edu.pl

Dr Małgorzata Benedyk-Machaczka, Assistant Professor
room: C225 (2.1.29), phone: 12 664 61 10, mail: malgorzata.benedyk@uj.edu.pl

Dr Danuta Bryzek, Research Assistant 
room: C243 (2.1.31), phone: 12 664 61 29, mail: danuta.bryzek@uj.edu.pl

Dr Ewelina Dobosz, Assistant Professor
room: C243 (2.1.31), phone: 12 664 61 29, mail: e.dobosz@uj.edu.pl

Dr Anna Golda, Assistant Professor
room: B219 (3.1.23), phone: 12 664 63 77, mail: anna.b.golda@uj.edu.pl

Dr Aleksander Grabiec, Assistant Professor
room: B243 (3.1.32), phone: 12 664 65 40, mail: aleksander.grabiec@uj.edu.pl, strona: www.epiperio.wbbib.uj.edu.pl

Dr Mirosław Książek, Research Assistant 
room: B203 (3.1.37), phone: 12 664 63 78, mail: książek.miroslaw@uj.edu.pl

Dr Jakub Kwieciński, Assistant Professor
room: C243 (2.1.31), phone: 12 664 61 29, mail: jakub1.kwiecinski@uj.edu.pl

Dr inż. Mariusz Madej, Assistant Professor
room: 1.01.4, phone: 12 664 61 24, mail: mariusz.madej@uj.edu.pl

Dr Stanisław Malicki, Assistant Professor
room: 1.01.4, phone: 12 664 61 24, mail: stanislaw.malicki@uj.edu.pl

Dr Danuta Mizgalska, Assistant Professor
room: B219 (3.1.23), phone: 12 664 63 77, mail: danuta.mizglaska@uj.edu.pl

Dr Magdalena Pilarczyk- Żurek, Assistant Professor
room: C243 (2.1.31), phone: 12 664 61 29, mail: magdalena.pilarczyk-zurek@uj.edu.pl

Dr Maja Sochalska, Assistant Professor
room: B243 (3.1.32), phone: 12 664 65 40, mail: maja.sochalska@uj.edu.pl 

Dr Florian Veillard, Assistant Professor
room: B219 (3.1.23), phone: 12 664 63 77, mail: florian.veillard@uj.edu.pl

Dr Kinga Wójcik, Senior Lecturer
room: B201 (3.1.38), phone: 12 664 61 68, mail: kinga.wójcik@uj.edu.pl

Dr Irena Waligórska, Post-doctoral Researcher
room: A237 (4.1.12), phone: 12 664 65 35, mail: irena.waligorska@ uj.edu.pl

Mgr Marta Kossowicz-Reroń, 
room: C225 (2.1.29), phone: 12 664 61 10, mail: marta.kossowicz-reron@uj.edu.pl

Mgr Magdalena Nowak, Senior Technical Specialist
room: B219 (3.1.23), phone: 12 664 63 77, mail: magdalena.lidia.nowak@uj.edu.pl

Mgr Agata Potempa, 
room: B203 (3.1.37), phone: 12 664 63 78, mail: agata.potempa@uj.edu.pl 

Mgr Aneta Sroka, Technical Assistant
room: B203 (3.1.37), phone: 12 664 63 78, mail: aneta.sroka@uj.edu.pl

Joanna Budziaszek, room: C241 (2.1.33), phone: 12 664 62 43, mail: joanna.budziaszek@doctoral.uj.edu.pl
Dominika Drapała, room: C226 (2.1.30), phone: 12 664 63 87, mail: dominika.drapala@doctoral.uj.edu.pl
Kamila Drzazga, room: C241 (2.1.33), phone: 12 664 62 43, mail: kamila.drzazga@doctoral.uj.edu.pl
Anna Jacuła, room: A237 (4.1.12), phone: 12 664 65 35, mail: anna.jacula@doctoral.uj.edu.pl
Michał Kanoza, room: C226 (2.1.30), phone: 12 664 63 87, mail: michal.kanoza@doctoral.uj.edu.pl
Weronika Kowaczuk, room: C241 (2.1.33), phone: 12 664 62 43, mail: nika.kovalchuk@doctoral.uj.edu.pl
Dominik Kowalczyk, room: C226 (2.1.30), phone: 12 664 63 87, mail: dominik.kowalczyk@doctoral.uj.edu.pl
Katarzyna Mikruta, pokój: C226 (2.1.30), telefon: 12 664 63 87, e-mail: michal.kanoza@doctoral.uj.edu.pl
Nazlı Ece Özçatalkaya, room: C226 (2.1.30), phone: 12 664 63 87, e-mail: nazli.ozcatalkaya@doctoral.uj.edu.pl
Elwira Nieboga, room: C226 (2.1.30), phone: 12 664 63 87, mail: elwira.nieboga@doctoral.uj.edu.pl
Alicja Płonczyńska, room: C226 (2.1.30), phone: 12 664 63 87, mail: alicja.plonczynska@doctoral.uj.edu.pl
Aureliusz Schuster, room: C226 (2.1.30), phone: 12 664 63 87, mail: aureliusz.schuster@doctoral.uj.edu.pl
Marta Słomka, room: C241 (2.1.33), phone: 12 664 62 43, mail:marta.slomka@doctoral.uj.edu.pl 
Natalia Stechnij, room: B203 (3.1.37), phone: 12 664 63 78, mail: natalia.stechnij@doctoral.uj.edu.pl
Aleksandra Wiekiera, room: C241 (2.1.33), phone: 12 664 62 43, mail: aleksandra.wiekiera@doctoral.uj.edu.pl
Mariola Wolska-Gębarzewska, room: B205 (3.1.35), phone: 12 664 63 65, mail: mariola.wolska@doctoral.uj.edu.pl
Natalia Zubrzycka, room: B243 (3.1.32), phone: 12 664 65 40, mail: natalia.zubrzycka@doctoral.uj.edu.pl

  • Pathogenesis of autoimmune diseases, including rheumatoid arthritis
  • Role of posttranslational modifications in immune system regulation
  • Role of negative inflammatory regulators in bacterial infection
  • Role of kallikrein-related proteases in EGF-related signal transduction
  • Role of arginine citrullination in the protease-inhibitor interactions in the context of innate immunity system
  • Role of citrullination in healing and regeneration of periodontal tissues
  • Defense mechanisms of bacteria
  • Epigenetics of bacterial infections
  • Apoptosis of immune cells and its role in the development of periodontitis 
  • Molecular mechanisms of bacterial opportunistic infections
  • Genetic and phenotypic bacterial strains typing
  • Pathogen transmission between human and animal
  • Advanced molecular diagnostics for bacterial infections 
  • Next-generation sequencing (NGS) in medical microbiology
  • The role of fibroblast in the pathogenesis of periodontitis
  • Significance of citrullination catalyzed by PPAD in the pathogenesis of periodontitis
  • Proteases and their protein inhibitors
  • Mechanisms underlying clinical and epidemiological connections between periodontitis and systemic human diseases in the context of dysbiotic oral microbiota
  • Understanding the structure, functions, and significance of the bacterial type nine secretion system (T9SS)
  • Molecular mechanisms of the importance of essential proteins in the physiology of bacterial cells
  • Molecular mechanisms of particle transport through bacterial cell membranes
  • Identification of novel antimicrobial compounds

  • Models of in vitro and in vivo infections (subcutaneous chamber model, paradontosis model, model of aspiration pneumonia, model of rheumatoid arthritis, sepsis model, skin infection model)
  • Organotypic model of the human gingiva
  • Flow cytometry
  • Cell death analysis, cytotoxicity, cell proliferation, differentiation
  • Primary tissue cultures/organotypic cultures of gingival mucosa
  • In vitro cultures of progenitors and neutrophils immortalized with the HoxB8 transcription factor
  • Histology, immunomorphology, immunohistology and immunohistochemistry of tissues
  • Computer analysis of immunofluorescent stained cells and tissues
  • Fluorescence microscopy
  • Immunofluorescence microscopy
  • Biochemical baterial strains typing
  • Genetic methods in bacteria typing
  • Next generation sequencing (WGS, RNASeq)
  • Gene expression analysis (qPCR)
  • Protein analysis (ELISA, Western Blot)
  • Elektrophoresis 2DE, proteins and DNA electrophoresis
  • Chromatography (FPLC, HPLC, LC-MS)
  • Computed tomography
  • Cryo-electron microscopy (cryo-EM)
  • X-ray crystallography

  1. Jakub Kwieciński: The central role of human hemostatic system in formation of Staphylococcus aureus biofilms. OPUS 25 (2024–2027), NCN
  2. Jakub Kwieciński: Together or not? The relevance of the interplay of staphylocoagulase and staphylokinase in the development of Staphylococcus aureus infections. OPUS 24 (2023–2027), NCN
  3. Mirosław Książek: The structures, biochemistry, physiology, and evolution of bacterial α2-macroglobulins from the human periodontopathogens Porphyromonas gingivalis and Tannerella forsythia. Sonata Bis 11 (2022–2027), NCN
  4. Amir Aliramezani: Characterization of the neutrophils’ role in controlling and shaping the microbiota. MINIATURA 7 (2023–2024), NCN
  5. Amir Aliramezani: Repurposing of existing drugs for treatment of canine Prototheca infections, Fellowship Training (2024), Morris Animal Foundation
  6. Aleksander Grabiec: The role of histone-modifying enzymes in the epigenetics and pathogenesis of. Periodontitis. OPUS 22 (2022–2026), NCN
  7. Ewelina Dobosz: Porphyromonas gingivalis opens new gates for virus infection – how periodontitis abolishes antiviral response. Sonata 17 (2022–2025), NCN
  8. Małgorzata Benedyk-Machaczka: Single-stranded DNA aptamer capable of specifically binding human PD-L1 as a new molecular probe in cancer diagnosis. SGS, (2022–2024), NCBR
  9. Alicja Płonczyńska: Przeciwzapalne działanie senoterapeutyków na fibroblasty dziąsłowe jako potencjalna terapia choroby zapalnej przyzębia, PRELUDIUM (2022–2024), NCN
  10. Danuta Bryzek: Neutrophils heterogeneity based on serine proteases, Sonata Bis 10 (2021–2026), NCN
  11. Maja Sochalska: Targeting deregulated expression of anti-apoptotic proteins belonging to the Bcl-2 family in macrophages as a potential therapy against periodontal disease. SONATA 16 (2021–2025), NCN
  12. Natalia Zubrzycka: Role of neutrophil cell death in the pathobiology of periodontitis, PRELUDIUM (2021–2024), NCN
  13. Piotr Mydel: Platelets in the Uremic Milieu – The link between protein carbamylation and platelet dysfunctions in end stage renal disease, OPUS 17 (2020–2024), NCN
  14. Mirosław Książek: The KLIKK proteases of human pathogen Tannerella forsythia: putative virulence factors with unique biochemical and structural properties. OPUS-18 (2020–2024), NCN
  15. Anna Golda: Therapeutic application of temporin derivatives in infectious diseases caused by intracellular pathogens. SONATA 15 (2020–2024), NCN
  16. Aleksander Grabiec: Synergistic activation of gingival fibroblasts by oral pathogens and inflammatory tissue environment as a new pathomechanism of chronic inflammation in periodontitis. OPUS 18 (2020–2024), NCN
  17. Joanna Kozieł: Identification of Streptococcus anginosus virulence factors, OPUS 15 (2019–2024), NCN
  18. Jan Potempa: Alzheimer’s disease as a co-morbidity of chronic periodontitis with Porphyromonas gingivalis as a causative link between both diseases. JPND (2019– 2024), NCN
  19. Joanna Kozieł: The reins of NETs – identification of mechanism controlling inflammatory potential of NETs. SONATA BIS (2017–2024), NCN

  1. Organotypic model of the gingiva for studying bacterial and viral pathogens implicated in periodontitis. Golda A, Gasiorek A, Dobosz E, Oruba Z, Lamont RJ, Potempa J, Koziel J. J Oral Microbiol. 2024 16: 292382. DOI: 10.1080/20002297.2023.2292382.
  2. The Bacteroidetes Q-rule and glutaminyl cyclase activity increase the stability of extracytoplasmic proteins. Szczęśniak K, Veillard F, Scavenius C, Chudzik K, Ferenc K, Bochtler M, Potempa J, Mizgalska D. mBio. 2023 Oct 31;14(5):e0098023. doi: 10.1128/mbio.00980-23.
  3. Identification and characterization of aptameric inhibitors of human neutrophil elastase. Malicki S, Książek M, Sochaj Gregorczyk A, Kamińska M, Golda A, Chruścicka B, Mizgalska D, Potempa J, Marti HP, Kozieł J, Wieczorek M, Pieczykolan J, Mydel P, Dubin G. J Biol Chem. 2023 Aug;299(8):104889. doi: 10.1016/j.jbc.2023.104889.
  4. Porphyromonas gingivalis Peptidyl Arginine Deiminase (PPAD) in the Context of the Feed-Forward Loop of Inflammation in Periodontitis. Prucsi Z, Zimny A, Płonczyńska A, Zubrzycka N, Potempa J, Sochalska M. Int J Mol Sci. 2023 Aug 18;24(16):12922. doi: 10.3390/ijms241612922.
  5. Bestatin as a treatment modality in experimental periodontitis. Kaminska M, Benedyk-Machaczka M, Adamowicz K, Aliko A, Drzazga K, Słysz K, Bielecka E, Potempa J, Mydel P. J Periodontol. 2023 Nov;94(11):1338-1350. doi: 10.1002/JPER.22-0614.
  6. The Role of Gingival Fibroblasts in the Pathogenesis of Periodontitis. Wielento A, Lagosz-Cwik KB, Potempa J, Grabiec AM. J Dent Res. 2023 May;102(5):489-496. doi: 10.1177/00220345231151921.
  7. Depletion of Mcpip1 in murine myeloid cells results in intestinal dysbiosis followed by allergic inflammation. Szukala W, Pilarczyk-Zurek M, Folkert J, Kotlinowski J, Koziel J, Jura J. Biochim Biophys Acta Mol Basis Dis. 2023 May 29;1869(7):166764. 
  8. Studies of Streptococcus anginosus Virulence in Dictyostelium discoideum and Galleria mellonella Models. Budziaszek J, Pilarczyk-Zurek M, Dobosz E, Kozinska A, Nowicki D, Obszanska K, Szalewska-Pałasz A, Kern-Zdanowicz I, Sitkiewicz I, Koziel J. Infect Immun. 2023 Apr 25:e0001623. doi: 10.1128/iai.00016-23. Epub ahead of print. PMID: 37097148.
  9. MCP-Induced Protein 1 Participates in Macrophage-Dependent Endotoxin Tolerance.Wadowska M, Dobosz E, Golda A, Bryzek D, Lech M, Fu M, Koziel J. J Immunol. 2022 Oct 1;209(7):1348-1358. doi: 10.4049/jimmunol.2101184. Epub 2022 Aug 31. PMID: 36165203.
  10. The Clinical View on Streptococcus anginosusGroup - Opportunistic Pathogens Coming Out of Hiding. Pilarczyk-Zurek M, Sitkiewicz I, Koziel J. Front Microbiol. 2022 Jul 8;13:956677. 
  11. Mapping of DNA methylation-sensitive cellular processes in gingival and periodontal ligament fibroblasts in the context of periodontal tissue homeostasis. Lagosz-Cwik KB, Melnykova M, Nieboga E, Schuster A, Bysiek A, Dudek S, Lipska W, Kantorowicz M, Tyrakowski M, Darczuk D, Kaczmarzyk T, Gilijamse M, de Vries TJ, Potempa J, Grabiec AM. Front Immunol. 2023 Jan 26;14:1078031. doi: 10.3389/fimmu.2023.1078031.
  12. The subversion of toll-like receptor signaling by bacterial and viral proteases during the development of infectious diseases. Ciaston I, Dobosz E, Potempa J, Koziel J. Mol Aspects Med. 2022 Sep 21;88:101143.
  13. Response regulator PorX coordinates oligonucleotide signalling and gene expression to control the secretion of virulence factors. Schmitz C, Madej M, Nowakowska Z, Cuppari A, Jacula A, Ksiazek M, Mikruta K, Wisniewski J, Pudelko-Malik N, Saran A, Zeytuni N, Mlynarz P, Lamont RJ, Usón I, Siksnys V, Potempa J, Solà M. Nucleic Acids Res. 2022 Nov 28;50(21):12558-12577. 
  14. Proteolytic Activity-Independent Activation of the Immune Response by Gingipains from Porphyromonas gingivalis. Ciaston I, Budziaszek J, Satala D, Potempa B, Fuchs A, Rapala-Kozik M, Mizgalska D, Dobosz E, Lamont RJ, Potempa J, Koziel J. mBio. 2022 Jun 28;13(3):e0378721.
  15. TLR2 Activation by Porphyromonas gingivalis Requires Both PPAD Activity and Fimbriae. Wielento A, Bereta GP, Łagosz-Ćwik KB, Eick S, Lamont RJ, Grabiec AM, Potempa J. Front Immunol. 2022 Apr 1;13:823685.
  16. May Staphylococcus lugdunensis Be an Etiological Factor of Chronic Maxillary Sinuses Infection? Kosecka-Strojek M, Wolska-Gębarzewska M, Podbielska-Kubera A, Samet A, Krawczyk B, Międzobrodzki J, Michalik M. Int J Mol Sci. 2022 Jun 9;23(12):6450.
  17. Uncovering the Oral Dysbiotic Microbiota as Masters of Neutrophil Responses in the Pathobiology of Periodontitis. Prucsi Z, Płonczyńska A, Potempa J, Sochalska M. Front Microbiol. 2021 Oct 11;12:729717.
  18. Intermolecular latency regulates the essential C-terminal signal peptidase and sortase of the Porphyromonas gingivalis type-IX secretion system. Mizgalska D, Goulas T, Rodríguez-Banqueri A, Veillard F, Madej M, Małecka E, Szczesniak K, Ksiazek M, Widziołek M, Guevara T, Eckhard U, Solà M, Potempa J, Gomis-Rüth FX. Proc Natl Acad Sci U S A. 2021 Oct 5;118(40):e2103573118.
  19. Latency, thermal stability, and identification of an inhibitory compound of mirolysin, a secretory protease of the human periodontopathogen Tannerella forsythia. Zak KM, Bostock MJ, Waligorska I, Thøgersen IB, Enghild JJ, Popowicz GM, Grudnik P, Potempa J, Ksiazek M. J Enzyme Inhib Med Chem. 2021 Dec;36(1):1267-1281.
  20. Subversion of Lipopolysaccharide Signaling in Gingival Keratinocytes via MCPIP-1 Degradation as a Novel Pathogenic Strategy of Inflammophilic Pathobionts. Gasiorek A, Dobosz E, Potempa B, Ciaston I, Wilamowski M, Oruba Z, Lamont RJ, Jura J, Potempa J, Koziel J. mBio. 2021 Jun 29;12(3):e0050221.
  21. hTERT-immortalized gingival fibroblasts respond to cytokines but fail to mimic primary cell responses to Porphyromonas gingivalis. Lagosz-Cwik KB, Wielento A, Lipska W, Kantorowicz M, Darczuk D, Kaczmarzyk T, Gibbs S, Potempa J, Grabiec AM. Sci Rep. 2021 May 24;11(1):10770.
  22. Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation. Dobosz E, Lorenz G, Ribeiro A, Würf V, Wadowska M, Kotlinowski J, Schmaderer C, Potempa J, Fu M, Koziel J, Lech M. Dis Model Mech. 2021 Mar 18;14(3):dmm047589.
  23. MCPIP-1 Restricts Inflammation via Promoting Apoptosis of Neutrophils. Dobosz E, Wadowska M, Kaminska M, Wilamowski M, Honarpisheh M, Bryzek D, Potempa J, Jura J, Lech M, Koziel J. Front Immunol. 2021 Feb 26;12:627922.
  24. PorZ, an Essential Component of the Type IX Secretion System of Porphyromonas gingivalis, Delivers Anionic Lipopolysaccharide to the PorU Sortase for Transpeptidase Processing of T9SS Cargo Proteins. Madej M, Nowakowska Z, Ksiazek M, Lasica AM, Mizgalska D, Nowak M, Jacula A, Bzowska M, Scavenius C, Enghild JJ, Aduse-Opoku J, Curtis MA, Gomis-Rüth FX, Potempa J. mBio. 2021 Feb 23;12(1):e02262-20.
  25. Distribution and antibiotic-resistance of different Staphylococcus species identified by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) isolated from the oral cavity. Garbacz K, Wierzbowska M, Kwapisz E, Kosecka-Strojek M, Bronk M, Saki M, Międzobrodzki J. J Oral Microbiol. 2021 Sep 26;13(1):1983322.
  26. Citrullination-Resistant LL-37 Is a Potent Antimicrobial Agent in the Inflammatory Environment High in Arginine Deiminase Activity. Bryzek D, Golda A, Budziaszek J, Kowalczyk D, Wong A, Bielecka E, Shakamuri P, Svoboda P, Pohl J, Potempa J, Koziel J. Int J Mol Sci. 2020 Nov 30;21(23):9126.
  27. Structural and functional insights into oligopeptide acquisition by the RagAB transporter from Porphyromonas gingivalis. Madej M, White JBR, Nowakowska Z, Rawson S, Scavenius C, Enghild JJ, Bereta GP, Pothula K, Kleinekathoefer U, Baslé A, Ranson NA, Potempa J, van den Berg B. Nat Microbiol. 2020 Aug;5(8):1016-1025.
  28. Plasmin inhibition by bacterial serpin: Implications in gum disease. Sochaj-Gregorczyk A, Ksiazek M, Waligorska I, Straczek A, Benedyk M, Mizgalska D, Thøgersen IB, Enghild JJ, Potempa J. FASEB J. 2020 Jan;34(1):619-630.
  29. HDAC3 Regulates Gingival Fibroblast Inflammatory Responses in Periodontitis. Lagosz KB, Bysiek A, Macina JM, Bereta GP, Kantorowicz M, Lipska W, Sochalska M, Gawron K, Kaczmarzyk T, Chomyszyn-Gajewska M, Fossati G, Potempa J, Grabiec AM. J Dent Res. 2020 Jan;99(1):98-106.
  30. Presence of egc-positive major clones ST 45, 30 and 22 among methicillin-resistant and methicillin-susceptible oral Staphylococcus aureus strains. Kwapisz E, Garbacz K, Kosecka-Strojek M, Schubert J, Bania J, Międzobrodzki J. Sci Rep. 2020 Nov 3;10(1):18889.
  31. Proteolytic processing and activation of gingipain zymogens secreted by T9SS of Porphyromonas gingivalis. Veillard F, Sztukowska M, Nowakowska Z, Mizgalska D, Thøgersen IB, Enghild JJ, Bogyo M, Potempa B, Nguyen KA, Potempa J. Biochimie. 2019 Jun 15. 
  32. Triggering NETosis via protease-activated receptor (PAR)-2 signaling as a mechanism of hijacking neutrophils function for pathogen benefits. Bryzek D, Ciaston I, Dobosz E, Gasiorek A, Makarska A, Sarna M, Eick S, Puklo M, Lech M, Potempa B, Potempa J, Koziel J. PLoS Pathog. 2019 May 20;15(5):e1007773. 
  33. BET Bromodomain Inhibitors Suppress Inflammatory Activation of Gingival Fibroblasts and Epithelial Cells From Periodontitis Patients. Maksylewicz A, Bysiek A, Lagosz KB, Macina JM, Kantorowicz M, Bereta G, Sochalska M, Gawron K, Chomyszyn-Gajewska M, Potempa J, Grabiec AM. Front Immunol. 2019 Apr 30;10:933. 
  34. Porphyromonas gingivalis in Alzheimer's disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Dominy SS, Lynch C, Ermini F, Benedyk M, Marczyk A, Konradi A, Nguyen M, Haditsch U, Raha D, Griffin C, Holsinger LJ, Arastu-Kapur S, Kaba S, Lee A, Ryder MI, Potempa B, Mydel P, Hellvard A, Adamowicz K, Hasturk H, Walker GD, Reynolds EC, Faull RLM, Curtis MA, Dragunow M, Potempa J. Sci Adv. 2019 Jan 23;5(1):eaau3333. 
  35. Development and Validation of a Reference Data Set for Assigning Staphylococcus Species Based on Next Generation Sequencing of 16S-23S rRNA Region. Kosecka-Strojek M, Sabat AJ, Akkerboom V, Becker K, van Zanten E, Wisselink G, Miedzobrodzki J, Kooistra-Smid M, Friedrich AW. Front Cell Infect Microbiol. 2019 Aug 7;9:278. 
  36. Development of a Reference Data Set for Assigning Streptococcus and Enterococcus Species Based on Next Generation Sequencing of 16S-23S rRNA Region. Kosecka-Strojek M, Sabat AJ, Akkerboom V, Kooistra-Smid M, Miedzobrodzki J, Friedrich AW. Antimicrob Resist Infect Control. 2019 Nov 15;8:178. doi: 10.1186/s13756-019-0622-3
  37. The impact of Porphyromonas gingivalis peptidylarginine deiminase on biofilm formation, invasion and transcriptional landscape of epithelial cells. Aliko A, Kamińska M, Bergum B, Gawron K, Benedyk M, Hellvard A, Jonsson R, Lamont RJ, Malicki S, Potempa J, Delaleu N, Mydel P. Sci Rep. 2018 Sep 20;8(1):14144. doi: 10.1038/s41598-018-32603-y.
  38. A Novel Biological Role for Peptidyl-Arginine Deiminases: Citrullination of Cathelicidin LL-37 Controls the Immunostimulatory Potential of Cell-Free DNA Wong A, Bryzek D, Dobosz E, Scavenius C, Svoboda P, Rapala-Kozik M, Lesner A, Frydrych I, Enghild J, Mydel P, Pohl J, Thompson PR, Potempa J, Koziel J. J Immunol. 2018 Apr 1;200(7):2327-2340. 
  39. The Bacteroidetes Q-Rule: Pyroglutamate in Signal Peptidase I Substrates. Bochtler M, Mizgalska D, Veillard F, Nowak ML, Houston J, Veith P, Reynolds EC, Potempa J. Front Microbiol. 2018 Mar 1;9:230. 
  40. The case for periodontitis in the pathogenesis of rheumatoid arthritis. Potempa J, Mydel P, Koziel J. Nat Rev Rheumatol. 2017 Oct;13(10):606-620. doi: 10.1038/nrrheum.2017.132. Epub 2017 Aug 24. Review. 

  • Identification of bacterial and virial virulence factors
  • The role of epigenetic mechanisms in pathogen-host interaction
  • Modulation of immunological response during infection
  • Potranslational protein modifications during inflammation
  • Characterization of new bacterial proteolytic enzymes and their protein inhibitors
  • The influence of apoptotic proteins on the function and survival of immune system cells in the development of periodontal inflammatory disease (in vitro, in vivo, ex vivo)
  • Genetic and enzymatic bacterial strains typing
  • Analysis of bacterial virulence and multidrug resistance genes