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Department of Plant Physiology and Biochemistry

WWW

www.zfibr.wbbib.uj.edu.pl/

Head

Professor Jerzy Kruk
room: B221 (3.1.25), phone: +48 12 664 63 61

Staff

Prof. dr hab. Kazimierz Strzałka, profesor emeritus
room: A222 (4.1.24), phone: +48 48 12 664 65 09, e-mail: kazimierz.strzalka@uj.edu.pl

Prof. dr hab. Leszek Fiedor 
room: B224 (3.1.31), phone: +48 12 664 63 58, e-mail:leszek.fiedor@uj.edu.pl

Dr hab. Dariusz Latowski
room: A225 (4.1.27), phone: +48 12 664 65 36, e-mail: dariusz.latowski@uj.edu.pl

Dr hab. Przemysław Malec 
room: A221 (4.1.23), phone: +48 12 664 65 20, e-mail:przemyslaw.malec@uj.edu.pl

Dr hab. Beata Myśliwa-Kurdziel 
room: B227 (3.1.31), phone: +48 12 664 63 64, e-mail: b.mysliwa-kurdziel@uj.edu.pl

Dr Monika Bojko 
room: B225 (3.1.29), phone: +48 12 664 63 68, e-mail: m.bojko@uj.edu.pl

Dr Michał Gabruk 
room B222 (3.1.26), phone: +48 12 664 63 72, e-mail: michal.gabruk@uj.edu.pl

Dr Małgorzata Jemioła-Rzemińska
room: B222 (3.1.26), phone: +48 12 664 6537, e-mail: malgorzata.jemiola-rzeminska@uj.edu.pl

Dr Beatrycze Nowicka 
room: B226 (3.1.30), phone: +48 12 664 63 66, e-mail: beatrycze.nowicka@uj.edu.pl

Dr Paweł Jedynak 
room: A220  (4.1.22), phone: +48 12 664 65 45, e-mail: pawel.jedynak@uj.edu.pl

Dr Kinga Kłodawska 
room: A226 (4.1.28), phone: +48 12 664 65 34, e-mail: kinga.klodawska@uj.edu.pl

Dr Magdalena Kędra 
room: A223 (4.1.25), phone: +48 12 664 65 19, e-mail: magdalena.keDra@uj.edu.pl

Mgr inż. Anna Kowalczyk 
room: A226 (4.1.28), phone: +48 12 664 65 34, e-mail: kowalczyk.kowalczyk@uj.edu.pl

Mgr Dorota Kutrzeba 
room: A223 (4.1.25), phone: +48 12 664 65 19, e-mail: dorota.kutrzeba@uj.edu.pl

Mgr Bartosz Pluciński 
room: A220 (4.1.22), phone: +48 12 664 65 45, e-mail: bartosz.plucinski@uj.edu.pl

Mgr Weronika Rajwa 
room: A230 (4.1.7), phone: +48 12 664 65 64, e-mail: weronika.rajwa@uj.edu.p

 

PhD students

Emilia Capała, room: A230 (4.1.7), phone: +48 12 664 65 64
Stanisław Listwan, room: A230 (4.1.7), phone: +48 12 664 65 64
Jan Pukalski, room: A220 (4.1.22), phone: +48 12 664 65 45
Wiktor Tokarek, room: A230 (4.1.7), phone: +48 12 664 65 64
Kamil Trzebuniak, room: A230 (4.1.7), phone: +48 12 664 65 64
Martyna Wasilewska, room: A230 (4.1.7), phone: +48 12 664 65 64
Mateusz Zbyradowski, room: A230 (4.1.7), phone: +48 12 664 65 64

Research topics

  • The xanthophyll cycle of higher plants, green algae and diatoms; analysis of de-epoxidation in thylakoid membranes and in artificial membrane systems
  • Protective role of carotenoids – molecular mechanism of epoxy xanthophylls de-epoxidation
  • The effect of heavy metals on the germination of seeds, physiology biochemistry and genetics of plants  from post-mining areas – in in vitro culture
  • Adaptations of plants to anthropogenic heavy metal stress: mechanisms and propagation
  • The role of antioxidants in the response to heavy metal stress 
  • Plants and microorganisms in bioremediation
  • Molecular mechanisms of response in autotrophic organisms to abiotic environmental factors (light, temperature, transition metals), including: environmental regulation of chlorophyll and carotenoid biosynthesis pathways, physiological function of oligomerization of photosystem I in cyanobacteria; plant metabolic adaptations to elevated concentrations of metal ions
  • Application of autotrophs to phytoremediation and biotransformation of hazardous wastes
  • Development of photosynthetic membranes in higher plants; chlorophyll biosynthesis
  • Physicochemical properties of biological membranes
  • Thermotropic phase transitions of lipids
  • Cyanobacterial cell wall metabolism

Methods

  • Liquid chromatography (HPLC-DAD, FPLC-DAD, HPTLC)
  • Gas chromatography (GC-FID)
  • PCR
  • EPR spectroscopy
  • Absorption spectroscopy
  • Fluorescence spectroscopy (steady-state and time-resolved methods at room temperature and at 77K, fluorescence anizotropy)
  • Electrophoresis
  • Isolation of plastid membranes
  • Pigment extraction from plant tissue
  • Electronic absorption and emission spectroscopy
  • Circular dichroism
  • Quantum-chemical computation
  • Plant and cell culture
  • Protein expression and purification using E.coli expression system
  • Stable transformation of A. thaliana with Agrobacterium

Current projects

  1. Leszek Fiedor: Chlorophylls and carotenoids under pressure – spectroscopy and high pressure in investigation of primary photosynthetic process. (2020-2022) 2019/33/B/NZ1/02418, OPUS 17, National Science Centre (NCN).
  2. Jerzy Kruk: Modification of plastoquinone :side-chain length as a method of increasing plant photosynthesis efficiency (2016-2019 ) 2015/19/B/NZ9/00422, OPUS10, National Science Centre (NCN)
  3. Beata Myśliwa-Kurdziel: The role of light-dependent chlorophyll biosynthesis in the regulation of biosynthesis and the assembly of photosynthetic dye-protein-lipid complexes in flowering plants. (2014-2018).2013/10/E/NZ3/00748 SONATA BIS, National Science Centre (NCN). 

Selected publications

  1. Aleku, G. A., Nowicka, B., & Turner, N. J. (2018). Biocatalytic Potential of Enzymes Involved in the Biosynthesis of Isoprenoid Quinones. ChemCatChem, 10, 124-135.
  2. Bojko, M., Olchawa-Pajor, M., Goss, R., Schaller-Laudel, S., Strzałka, K., & Latowski, D. (2019). Diadinoxanthin de-epoxidation as important factor in the short-term stabilization of diatom photosynthetic membranes exposed to different temperatures. Plant, Cell & Environment, 42, 1270-1286.
  3. Budziak, I., Karcz, D., Makowski, M., Myśliwa-Kurdziel, B., Kasprzak, K., Matwijczuk, A. & Matwijczuk, A. (2019). Spectroscopic and theoretical investigation into substituent-and aggregation-related dual fluorescence effects in the selected 2-amino-1, 3, 4-thiadiazoles. Journal of Molecular Liquids, 291, e111261.
  4. Fiedor, L., Dudkowiak, A. & Pilch, M. (2019). The origin of the dark S1 state in carotenoids: a comprehensive model. Journal of the Royal Society Interface, 16, e20190191.
  5. Gabruk, M., Myśliwa-Kurdziel, B. & Kruk, J. (2017). MGDG, PG and SQDG regulate the activity of light-dependent protochlorophyllide oxidoreductase. Biochemical Journal, 474, 1307 – 1320.
  6. Kłodawska, K., Bujas, A., Turos-Cabal, M., Żbik, P., Fu, P. & Malec, P. (2019). Effect of growth temperature on biosynthesis and accumulation of carotenoids in cyanobacterium Anabaena sp. PCC 7120 under diazotrophic conditions. Microbiological Research, 226, 34-40.
  7. Kłodawska, K., Kovács, L., Vladkova, R., Rzaska, A., Gombos, Z., Laczkó-Dobos, H., & Malec, P. (2019). Trimeric organization of photosystem I is required to maintain the balanced photosynthetic electron flow in cyanobacterium Synechocystis sp. PCC 6803. Photosynthesis Research, in press.
  8. Kovacs, T., Szalontai, B., Kłodawska, K., Vladkova, R., Malec, P., Gombos, Z., & Laczko-Dobos, H. (2019). Photosystem I oligomerization affects lipid composition in Synechocystis sp. PCC 6803. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids, 1864, 1384-1395.
  9. Kowalczyk, A. & Latowski, D. (2018). Role of Plant-Microorganism Interactions in Plant Tolerance to Arsenic. In Mechanisms of Arsenic Toxicity and Tolerance in Plants , 219-237, Springer, Singapore.
  10. Lang, J., Melnykova, M., Catania, M., Inglot, A., Zyss, A., Mikruta, K., ... & Nalepa, P. (2019). A water-soluble [60] fullerene-derivative stimulates chlorophyll accumulation and has no toxic effect on Chlamydomonas reinhardtii. Acta Biochimica Polonica, 66, 257-262.
  11. Lin, T. Y., Abbassi, N. E. H., Zakrzewski, K., Chramiec-Głąbik, A., Jemioła-Rzemińska, M., Różycki, J., & Glatt, S. (2019). The Elongator subunit Elp3 is a non-canonical tRNA acetyltransferase. Nature Communications, 10, e625.
  12. Michalik, M., Zbyradowski, M., & Fiedor, L. (2019). Tuning the Photophysical Features of Self-Assembling Photoactive Polypeptides for Light-Harvesting. Materials, 12, e3554.
  13. Nowicka B. (2019) Target genes for plant productivity improvement, Journal of Biotechnology 298: 21-34.
  14. Nowicka, B. (2019). Practical aspects of the measurements of non-photochemical chlorophyll fluorescence quenching in green microalgae Chlamydomonas reinhardtii using Open FluorCam. Physiologia Plantarum, in press.
  15. Olchawa-Pajor, M., Bojko, M., Strzałka, W., Strzałka, K., & Latowski, D. (2019). Violaxanthin conversion by recombinant diatom and plant de-epoxidases, expressed in Escherichia coli – comparative analysis. Acta Biochimica Polonica, 66, 249-255.
  16. Szymańska R., Nowicka B., Trela A., Kruk J. (2019) Vitamin E: structure and forms, in: Molecular Nutrition Vitamins (Ed. V. P. Patel), Academic Press UK, 67-90, https://doi.org/10.1016/B978-0-12-811907-5.00021-X.
  17. Trela, A., Silska, G., Chyc, M., Latowski, D., Kruk, J., & Szymańska, R. (2019). Tocochromanols and fatty acid composition in flax (Linum usitatissimum L.) accessions. Acta Societatis Botanicorum Poloniae, 88, e3636.
  18. Vaňousová, K., Beranová, J., Fišer, R., Jemioła-Rzemińska, M., Lišková, P. M., Cybulski, L., & Konopásek, I. (2018). Membrane fluidization by alcohols inhibits DesK-DesR signalling in Bacillus subtilis. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1860, 718-727.

 

Batchelor/master thesis topics

  • Photosynthesis under stress conditions – adaptations and protective mechanisms; xanthophyll cycle; formation of the photosynthetic apparatus, light-dependent photosynthetic reactions
  • Investigation of primary photosynthetic reactions and interactions between photosynthetic complexes in model systems; physical-chemical properties of thylakoid membranes; modifications of photosynthetic pigments in terms of application in photodynamic therapy; carotenoids and their role in photosynthesis process
  • Reconstitution of photosynthetic protein pigment complexes; pro-oxidant  properties of carotenoids
  • Plant metabolites and their role in medicine; artificial membranes; redox reactions in chloroplasts
  • Analysis of chlorophyllide, protochlorophyllide and protochlorophyll photophysical properties in model systems; structures of photoactive complexes protochlorophyllide: oxidoreductase, protochlorophyllide: NADPH – using fluorescence quenching technique
  • Effect of selected heavy metals on light-dependent photosynthetic reactions of higher plants on different levels of organization – individual and evolutionary aspects
  • Plants and microorganisms in bioremediation of contaminated areas – molecular aspects, practical application
  • Fluorescent properties of reduced prenyl quinones and their application in in vitro research
  • The role of prenyllipids in plant stress response 
  • Formation and quenching of ROS in photosynthesis
  • Acclimatization of diatoms to environmental changes  
  • The role of low-molecular weight antioxidants and antioxidant enzymes in the response to heavy metal-induced stress 
  • Biochemical characterization of selected enzymes involved in peptidoglycan metabolism in cyanobacteria

Requirements for candidates

Knowledge on physiology and biochemistry of plants, molecular biology, physics and chemistry. Fluent English. Laboratory skills.