www.zbtr.wbbib.uj.edu.pl

Dr hab. Wojciech Strzałka
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: wojciech.strzalka@uj.edu.pl

Prof. dr hab. Halina Gabryś, emeritus
room: B223 (3.1.27), phone: +48 12 664 63 40, e-mail: halina.gabrys@uj.edu.pl

Dr. Agnieszka K. Banaś
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: a_katarzyna.banas@uj.edu.pl

Dr. Ewa Kowalska
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: ewa.b.kowalska@uj.edu.pl

Dr. Weronika Krzeszowiec-Jeleń
room: B223 (3.1.27), phone: +48 12 664 63 40, e-mail: weronika.krzeszowiec@uj.edu.pl

Dr. Jan Łyczakowski
room: B105 (3.0.33), phone: + 48 12 664 63 47, e-mail: jan.lyczakowski@uj.edu.pl 

Dr. Arkadiusz Borek
room: A220 (4.1.22), phone: + 48 12 664 65 45, e-mail: arkadiusz.borek@uj.edu.pl 

Dr. Piotr Zgłobicki
room: B105 (3.0.33), phone: + 48 12 664 63 47, e-mail: piotr.zglobicki@uj.edu.pl

Mgr Maria Niemczura 
room: B232 (3.1.8), phone: + 48 12 664 63 91, e-mail: maria.niemczura@uj.edu.pl

Aneta Bażant, room: B105 (3.0.33), phone + 48 12 664 63 47
Aleksandra Liszka, room: B105 (3.0.33), phone +48 12 664 63 47
Aleksandra Lewandowska, room: A220 (4.1.22), phone: +48 12 664 65 45
Katarzyna Leja, room: A220 (4.1.22), phone: +48 12 664 65 45

• Proteins engaged in DNA metabolism (PCNA, Fen1, photolyases) studies
• Signaling role of plant kinases 
• The role of post-transcriptional and post-translational modifications in plant stress response 
• Applications of DNA aptamers in molecular biology, biotechnology and medicine
• Photoreceptor role in plant functioning
• Identification of signals originating from chloroplasts which regulate their movements
• Signaling role of basic metabolites in phototropin-controlled chloroplast responses
• Second messengers of blue light signals, phosphoinositides and Ca²⁺, in chloroplast responses. 
• Involvement of plant GLR channels in blue-light controlled growth and movement response

• Plant cell and tissue culture
• Photometric and microscopic analysis of organelle movements 
• Fluorescence and confocal microscopy
• Bacteria and yeast transformation
• Stable and transient transformation of plants using Agrobacterium and biolistic method (gene gun)
• Protein analysis: ELISA, Western blotting, SDS page, chromatography
• Protein interaction: yeast one/two-hybrid system, BIFC, pull-down, co-immunoprecipitation,
• DNA damage analysis: ELISA
• mRNA and DNA analyses: real-time PCR, Southern blotting
• Ca²⁺ luminometry based on aequorin expressing transgenic plants

1. Wojciech Strzałka: Analysis of the functions of isoforms of plant FEN1 and their phosphorylation using Arabidopsis thaliana as an experimental model. (2020–2023) OPUS 17, National Science Centre (NCN).
2. Jan Łyczakowski: Environment driven variation in the molecular architecture of softwood. (2019–2022). SONATINA 3, National Science Centre (NCN).
3. Agnieszka K. Banaś: Arabidopsis photolyases: the role of post-transcriptional and post-translational modifications, influence on DNA repair, chloroplast functioning and plant responses to abiotic stresses. (2017–2022). SONATA BIS 6, National Science Centre (NCN).
4. Halina Gabryś: The signaling role of basic metabolites in the phototropin-controlled chloroplast responses. (2017–2020). OPUS 12, National Science Centre (NCN).

1. Eckstein A, Grzyb J, Hermanowicz P, Zgłobicki P, Łabuz J, Strzałka W, Dziga D, Banaś AK. Arabidopsis phototropins participate in the regulation of dark-induced leaf senescence. International Journal of Molecular Sciences. 2021, 22(4), 1836; 
2. Łabuz J, Sztatelman O, Jagiełło-Flasińska D, Hermanowicz P, Bażant A, Banaś AK, F, Giza A, Kozłowska A, Lasok H, Sitkiewicz E, Krzeszowiec W, Gabryś H, Strzałka W. Phototropin interactions with SUMO proteins. Plant and Cell Physiology, pcab027,
3. Banaś AK, Zgłobicki P, Kowalska E, Bażant A, Dziga D, Strzałka W. All You Need Is Light. Photorepair of UV-Induced Pyrimidine Dimers. Genes (Basel). 2020 Nov 4;11(11):1304. doi: 10.3390/genes11111304. PMID: 33158066; PMCID: PMC7694213.
4. Strzałka W, Zgłobicki P, Kowalska E, Bażant A, Dziga D, Banaś AK. The Dark Side of UV-Induced DNA Lesion Repair. Genes (Basel). 2020 Dec 2;11(12):1450. doi: 10.3390/genes11121450. PMID: 33276692; PMCID: PMC7761550.
5. Kowalska E, Strzalka W, Takuji O. A crystallization and preliminary X-ray diffraction study of the Arabidopsis thaliana proliferating cell nuclear antigen (PCNA2) alone and in complex with a PIP-box peptide from Flap endonuclease 1. Acta Biochimica Polonica. 2020, Vol 67(1): 49-52  DOI: https://doi.org/10.18388/abp.2020_2896
6. Krzeszowiec W, Novokreshchenova M, Gabryś H. Chloroplasts in C3 grasses move in response to blue-light. Plant Cell Reports. 2020. doi: 10.1007/s00299-020-02567-3.
7. Kowalska E, Bartnicki F, Fujisawa R, Bonarek P, Hermanowicz P, Tsurimoto T, Muszyńska K, Strzalka W. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic Acid Research. 2018, 46:25-41. doi: 10.1093/nar/gkx1184.
8. Bartnicki F, Bonarek P, Kowalska E, Strzałka W. The Argi-system: one step purificaztion of proteins tagged with arginine-rich cell-penetrating peptides. Scientific Reports. 2017, volume  2619 DOI:10.1038/s41598-017-02432-6
9. Banaś AK, Hermanowicz P, Sztatelman O, Łabuz J, Aggarwal Ch, Zgłobicki P, Jagiełło-Flasińska D, Strzałka W. 6,4 - PP Photolyase Encoded by AtUVR3 is Localized in Nuclei, Chloroplasts and Mitochondria and Its Expression is Down-Regulated by Light in a Photosynthesis-Dependent Manner. Plant and Cell Physiology. 2017, pcx159, https://doi.org/10.1093/pcp/pcx159 
10. Muszyńska K, Ostrowska D, Bartnicki F, Kowalska E, Bodaszewska-Lubaś M, Hermanowicz P, Faulstich H, Strzałka W. Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides. Acta Biochimica Polonica. 2017, 64:401-406. doi: 10.18388/abp.2017_1615

The topics for B.Sc. and M.Sc. thesis are directly related to the current research lines that are pursued at our Department.

Basic knowledge of biochemistry, microbiology, molecular genetics, cell biology and plant physiology/biotechnology