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Department of Plant Biotechnology

Website

www.zbtr.wbbib.uj.edu.pl

Head

Dr hab. Wojciech Strzałka
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: wojciech.strzalka@uj.edu.pl

Staff

Prof. dr hab. Halina Gabryś, emeritus
room: B223 (3.1.27), phone: +48 12 664 63 40, e-mail: halina.gabrys@uj.edu.pl

Dr. Agnieszka K. Banaś
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: a_katarzyna.banas@uj.edu.pl

Dr. Ewa Kowalska
room: B242 (3.1.33), phone: +48 12 664 64 10, e-mail: ewa.b.kowalska@uj.edu.pl

Dr. Weronika Krzeszowiec-Jeleń
room: B223 (3.1.27), phone: +48 12 664 63 40, e-mail: weronika.krzeszowiec@uj.edu.pl

Dr. Jan Łyczakowski
room: B105 (3.0.33), phone: + 48 12 664 63 47 e-mail: jan.lyczakowski@uj.edu.pl 

Dr. Filip Bartnicki
room: B105 (3.0.33), phone: + 48 12 664 63 47 e-mail: filip.bartnicki@uj.edu.pl 

Dr. Anna Hebda
room: A220 (4.1.22), phone: +48 12 664 65 45 e-mail: a.hebda@uj.edu.pl

Mgr Maria Niemczura 
room: B232 (3.1.8), phone: + 48 12 664 63 91, e-mail: maria.niemczura@uj.edu.pl

PhD students

Klaudia Arciszewska, room: B105 (3.0.33), phone: +48 12 664 63 47
Aneta Bażant, room: B105 (3.0.33), phone + 48 12 664 63 47
Aleksandra Liszka, room: B105 (3.0.33), phone +48 12 664 63 4

 

Research topics

  • Proteins engaged in DNA metabolism (PCNA, Fen1, photolyases) studies
  • Signaling role of plant kinases 
  • The role of post-transcriptional and post-translational modifications in plant stress response 
  • Applications of DNA aptamers in molecular biology, biotechnology and medicine
  • Photoreceptor role in plant functioning
  • Identification of signals originating from chloroplasts which regulate their movements
  • Signaling role of basic metabolites in phototropin-controlled chloroplast responses
  • Second messengers of blue light signals, phosphoinositides and Ca2+, in chloroplast responses. 
  • Involvement of plant GLR channels in blue-light controlled growth and movement response

 

Methods

  • Plant cell and tissue culture
  • Photometric and microscopic analysis of organelle movements 
  • Fluorescence and confocal microscopy
  • Bacteria and yeast transformation
  • Stable and transient transformation of plants using Agrobacterium and biolistic method (gene gun)
  • Protein analysis: ELISA, Western blotting, SDS page, chromatography
  • Protein interaction: yeast one/two-hybrid system, BIFC, pull-down, co-immunoprecipitation,
  • DNA damage analysis: ELISA
  • mRNA and DNA analyses: real-time PCR, Southern blotting
  • Ca2+ luminometry based on aequorin expressing transgenic plants

Current projects

  1. Jan Łyczakowski: Environment driven variation in the molecular architecture of softwood. (2019-2022). SONATINA 3,  National Science Centre (NCN).
  2. Agnieszka Banaś: Arabidopsis photolyases: the role of post-transcriptional and post-translational modifications, influence on DNA repair, chloroplast functioning and plant responses to abiotic stresses. (2017-2022). SONATA BIS 6, National Science Centre (NCN).
  3. Halina Gabryś: The signaling role of basic metabolites in the phototropin-controlled chloroplast responses. (2017-2020). OPUS 12, National Science Centre (NCN).
  4. Wojciech Strzałka: Characterizing the functions of plant PCNA1 and PCNA2 proteins using Arabidopsis thaliana as an experimental model. (2014-2019). SONATA-BIS, National Science Centre (NCN).
  5. Anna Hebda: Ścieżka przekazu sygnału w odpowiedzi sosny zwyczajnej na stres zanieczyszczenia powietrza w krakowskiej aglomeracji miejskiej. (2019). MINIATURA 2, National Science Centre (NCN).

Selected publications

  1. Robson TM, Aphalo PJ, Banaś AK, Barnes PW, Brelsford CC, Jenkins GI, Kotilainen TK, Łabuz J, Martínez-Abaigar J, Morales LO, Neugart S, Pieristè M, Rai N, Vandenbussche F, Jansen MAK 2019. A perspective on ecologically relevant plant-UV research and its practical application. Photochemical & Photobiological Science
  2. Kowalska E, Bartnicki F, Fujisawa R, Bonarek P, Hermanowicz P, Tsurimoto T,   Muszyńska K, Strzalka W. 2018. Inhibition of DNA replication by an anti-PCNA aptamer/PCNA complex. Nucleic Acid Research 46:25-41.
  3. Banaś AK, Hermanowicz P, Sztatelman O, Łabuz J, Aggarwal Ch, Zgłobicki P, Jagiełło-Flasińska D, Strzałka W. 2018. 6,4 - PP photolyase encoded by AtUVR3 is localized in nuclei, chloroplasts and mitochondria and its expression is down-regulated by light in a photosynthesis-dependent manner. Plant and Cell Physiology 59 (1): 44-57, doi: 10.1093/pcp/pcx159
  4. Bartnicki F, Bonarek P, Kowalska E, Strzałka W. 2017. The Argi system: one-step purification of proteins tagged with arginine-rich cell-penetrating peptides. Scientific Reports (www.nature.com/scientificreports) 2017; vol. 7: 2619, 1-12, DOI:10.1038/s41598-017-02432-6
  5. Łabuz J, Samardakiewicz S, Hermanowicz P, Wyroba E, Pilarska M, Gabryś H. 2016. Blue light-dependent changes in loosely bound calcium in Arabidopsis mesophyll cells: an X-ray microanalysis study. Journal of Experimental Botany, doi:10.1093/jxb/erw089
  6. Sztatelman O, Łabuz J, Hermanowicz P, Banaś AK, Bażant A, Zgłobicki P, Aggarwal C, Nadzieja M, Krzeszowiec W, Strzałka W, Gabryś H. 2016. Fine tuning chloroplast movements through physical interactions between phototropins. Journal of Experimental Botany 67, 4963-4978, doi:10.1093/jxb/erw265. IF 5,677
  7. Eckstein A, Krzeszowiec W, Waligórski P, Gabryś H. 2015. Auxin and chloroplast movements. Physiologia Plantarum 105: 271-281.
  8. Sztatelman O, Grzyb J, Gabryś H, Banaś AK. 2015. The effect of UV-B on Arabidopsis leaves depends on light conditions after treatment. BMC Plant Biology 15:281.
  9. Strzałka W, Aggarwal C, Krzeszowiec W, Jakubowska A, Sztatelman O,  Banaś AK. 2015. Arabidopsis PCNAs form complexes with selected D-type cyclins. Frontiers in Plant Science 6:516.
  10. Aggarwal Ch, Banaś AK, Kasprowicz-Maluśki A, Borghetti C, Łabuz J, Dobrucki J, Gabryś H. 2014. Blue-light-activated phototropin2 trafficking from the cytoplasm to Golgi/post-Golgi vesicles. Journal of Experimental Botany 66 (12): 3263–3276.

Batchelor/master thesis topics

The topics for B.Sc. and M.Sc. thesis are directly related to the current research lines that are pursued at our Department.

Requirements for students

Basic knowledge of biochemistry, microbiology, molecular genetics, cell biology and plant physiology/biotechnology