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Department of Cell Biology

Webpage

http://www.zbk.wbbib.uj.edu.pl

Head

Prof. dr hab. Zbigniew Madeja
room: C120 (2.0.25), phone: +48 12 664 61 42, e-mail: z.madeja@uj.edu.pl

Staff

Prof. dr hab. Jarosław Czyż 
room: C+48 123 (2.0.28), phone: +48 +48 12 664 61 46, e-mail: jarek.czyz@uj.edu.pl

Dr hab. Justyna Drukała 
room: C+48 124 (2.0.29), phone: +48 +48 12 664 61 45, e-mail: justyna.Dr.ukala@uj.edu.pl

Dr hab. Marta Michalik 
room: C118 (2.0.23), phone: +48 +48 12 664 61 44, e-mail: marta.michalik@uj.edu.pl

Dr hab. Jolanta Sroka 
room: C+48 121 (2.0.26), phone: +48 +48 12 664 61 43, e-mail: jolanta.sroka@uj.edu.pl

Dr hab. Ewa Zuba-Surma 
room: C119A (2.0.24A), phone: +48 +48 12 664 61 80, e-mail: ewa.zuba-surma@uj.edu.pl

Dr. Sylwia Bobis-Wozowicz
room: C119B (2.0.24B), phone: +48 +48 12 664 62 27, e-mail: sylwia.bobis@uj.edu.pl

Dr. Paweł Ferdek 
room: C145 (2.0.33), phone: +48 +48 12 664 62 18, e-mail: pawel.ferdek@uj.edu.pl

Dr. Sławomir Lasota
room: C119B (2.0.24B), phone: +48 +48 12 664 62 27, e-mail:slawomir.lasota@uj.edu.pl

Dr. Katarzyna Piwowarczyk
room: C+48 122 (2.0.27), phone: +48 +48 12 664 61 83, e-mail: katarzyna.szpak@uj.edu.pl

Dr. Monika Rak 
room: C+48 122 (2.0.27), phone: +48 +48 12 664 61 83, e-mail: monika.rak@uj.edu.pl

Dr. Damian Ryszawy 
room: C+48 125 (2.0.30), phone: +48 +48 12 664 61 47, e-mail: damian.ryszawy@uj.edu.pl

Dr. Anna Łabędź-Masłowska 
room: C+48 122 (2.0.27), phone: +48 +48 12 664 61 83, e-mail: anna.labedz-maslowska@uj.edu.pl

Dr. Milena Paw 
room: C119B (2.0.24B), phone: +48 +48 12 664 62 27, e-mail: milena.paw@uj.edu.pl

Mgr Marcin Piejko 
room: C+48 129 (2.0.7), phone: +48 +48 12 664 6189, e-mail: marcin.piejko@gmail.com

Mgr Dawid Wnuk 
Cell Bank (2.0.8), phone +48 12 664 61 89, e-mail: dawid.wnuk@uj.edu.pl 

Mgr Lidia Ciastoń 
room: C137 (2.0.13), phone: +48 +48 12 664 61 40, e-mail: lidia.ciaston@uj.edu.pl

Mgr Jadwiga Golda 
room: C+48 125 (2.0.30), phone: +48 +48 12 664 61 47, e-mail: jadwiga.golda@uj.edu.p

 

PhD students

Jessica Catapano, room:  C125 (2.0.30), phone: +48 12 664 61 47
Katarzyna Kmiotek-Wasylewska, room: MCB 1/31
Marcin Luty, room:C122 (2.0.27), phone: +48 12 664 61 83
Sylwia Noga, room: MCB 1/31
Kinga Polańska, room: C119B (2.0.24B), phone: +48 12 66462 27
Joanna Stalińska, room: MCB 1/33
Agnieszka Szkaradek, room: C122 (2.0.27), phone: +48 12 664 61 83
Dawid Wnuk, room: C125 (2.0.30), phone: +48 12 664 61 47
Tomasz Wróbel, room:  C119B (2.0.24B), phone: +48 12 664 62 27

 

Research topics

  • Functions of direct cell-cell interactions in regulating cancer cell migration activity
  • Influence of the functions of gap junctions and cell adhesion molecules on the invasiveness of cancer cells
  • Role of reactive oxygen species in regulating the migration activity and intercellular communication by gap junctions
  • Electrotaxis mechanisms
  • Epithelial–mesenchymal transition (EMT) mechanisms
  • Application of electrochemical methods in cell biology
  • Fibroblast to myofibroblast transition  (FMT) mechanisms  and  role  of FMT in bronchial wall remodeling in asthma
  • Mechanisms of endocytosis
  • Application of in vitro skin cell cultures in clinical practice, such as wound treatment and plastic surgery
  • Tissue skin engineering – reconstruction of three-dimensional connective tissue equivalents using mesenchymal stem cells
  • Optimization of stem cell and progenitor cell populations for applications in experimental cardiology including induced pluripotent stem cells (iPS) as well as mesenchymal stem cells of different antigenic phenotype
    Use of stem cells and progenitor cells in regenerative medicine 
  • Cancer stem cells, cancer drug-resistance
  • Role of stem cell derived extracellular vesicles (native and genetically modified) in tissue regeneration
  • Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration
  • Development of optimized methods for treatment of tissue injuries based on innovative composites and mesenchymal stem cells and their derivatives in patients with civilization diseases

     

Methods and specialized equipment

  • Cell and tissue engineering
  • Fluorescence microscopy
  • Flow cytometry (Guava EasyCyte, Merck)
  • Water purification system Simplicity UV (Merk)
  • CFX384 Real Time System (BioRad) for gene expression analysis on 384 well plates
  • FACSAria III (Becton Dickinson) fluorescence-activated cell sorting system
  • Olympus IX81 fluorescent microscope equiped with MicroPublisher 3.3 RTV camera
  • Neon Electroporation System (Invitrogen) for cell electroporation
  • Measurement of nanoparticles concentration and size by Nanoparticle Tracking Analysis (NTA) (Nanosight NS300, Malvern)
  • Imaging flow cytometry (ImageStreamX Mark II Imaging Flow Cytometer, Merck)

Current projects

  1. Zbigniew Madeja: Biphasic mechanism of electrotaxis of mouse 3T3 fibroblasts: complementary role of ion channels and chemoattractant receptors. (2019-2022). OPUS 16, National Science Centre (NCN).
  2. Dawid Wnuk: The role of TGF-β1/Smad signalling pathway in the asthma-related fibroblast to myofibroblast transition. (2019-2020). ETIUDA 7, National Science Centre (NCN).
  3. Sylwia Bobis-Wozowicz: Impact of hypoxia on molecular characteristic and biological properties, including heart regenerative capacity of extracellular vesicles released by human induced pluripotent stem cells. (2017-2020). SONATA 12. National Science Centre (NCN).
  4. Katarzyna Kmiotek-Wasylewska: Prokardiomiogenne i proagiogenne właściwości mikropęcherzyków komórkowych pochodzących z ludzkich indukowanych pluripotencjalnych komórek macierzystych wykazujących nadekspresję wybranych mikroRNA. (2018-2019). ETIUDA 6, NCN
  5. Katarzyna Kmiotek: Optimization of parameters for evaluation of cardiomyogenesis efficacy in human cardiac mesenchymal stromal cells, during differentiation process, after treatment with human induced pluripotent stem cell derived extracellular vesicles. (2017-2019). PRELUDIUM 11, National Science Centre (NCN).
  6. Ewa Zuba-Surma: Development of optimized methods for treatment of tissue injuries based on innovative composites and mesenchymal stem cells and their derivatives in patients with civilization diseases. (2017-2020). STRATEGMED, National Centre for Research and Development (NCBiR) 
  7. Jarosław Czyż: Interference of fenofibrate with the invasive potential of prostate cancer cells modulated by chemotherapeutics. (2016-2019). OPUS 9, National Science Centre (NCN).
  8. Marta Michalik: Bronchial fibroblast to myofibroblast transition – the role of differential activation of TGF-b1/Smad signalling pathway in cells derived from asthmatic patients and non-asthmatic donors – in vitro studies in 2D, 3D models and model of the epithelial-mesenchymal trophic unit. (2016-2019). OPUS, National Science Centre (NCN).
  9. Damian Ryszawy: The role of Snail-1 and Cx43 in regulation of invasive properties of human glioblastoma multiforme. (2016-2019). SONATA 10, National Science Centre (NCN).
  10. Ewa Zuba-Surma: Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration. (2015-2019). SYMFONIA 3, National Science Centre (NCN).

Selected publications

  1. Adamiak M, Cheng G, Bobis-Wozowicz S, Zhao L, Kedracka-Krok S, Samanta A, Karnas E, Xuan YT, Skupien-Rabian B, Chen X, Jankowska U, Girgis M, Sekula M, Davani A, Lasota S, Vincent RJ, Sarna M, Newell KL, Wang OL, Dudley N, Madeja Z, Dawn B, Zuba-Surma EK. Induced Pluripotent Stem Cell (iPSC)-Derived Extracellular Vesicles Are Safer and More Effective for Cardiac Repair Than iPSCs. Circ Res. 2018 Jan 19;122(2):296-309. Epub 2017 Nov 8.
  2. Piwowarczyk K, Kwiecień E, Sośniak J, Zimoląg E, Guzik E, Sroka J, Madeja Z, Czyż J. Fenofibrate Interferes with the Diapedesis of Lung Adenocarcinoma Cells through the Interference with Cx43/EGF-Dependent Intercellular Signaling. Cancers (Basel). 2018 Sep 28;10(10). doi: 10.3390/cancers10100363.
  3. Sroka J, Zimolag E, Lasota S, Korohoda W, Madeja Z. Electrotaxis: Cell Directional Movement in Electric Fields. Methods Mol Biol. 2018;1749:325-340. doi: 10.1007/978-1-4939-7701-7_23.
  4. Zimolag E, Borowczyk-Michalowska J, Kedracka-Krok S, Skupien-Rabian B, Karnas E, Lasota S, Sroka J, Drukala J, Madeja Z. Electric field as a potential directional cue in homing of bone marrow-derived mesenchymal stem cells to cutaneous wounds. Biochim Biophys Acta Mol Cell Res. 2017 Feb;1864(2):267-279. doi: 10.1016/j.bbamcr.2016.11.011. Epub 2016 Nov 15. PubMed PMID: 27864076.
  5. Rak M, Ochałek A, Bielecka E, Latasiewicz J, Gawarecka K, Sroka J, Czyż J,Piwowarczyk K, Masnyk M, Chmielewski M, Chojnacki T, Swiezewska E, Madeja Z.Efficient and non-toxic gene delivery by anionic lipoplexes based on polyprenyl ammonium salts and their effects on cell physiology. J Gene Med. 2016 Nov;18(11-12):331-342. doi: 10.1002/jgm.2930. PubMed PMID: 27706881.
  6. Michalik M, Wójcik-Pszczoła K, Paw M, Wnuk D, Koczurkiewicz P, Sanak M, Pękala E, Madeja Z. Fibroblast-to-myofibroblast transition in bronchial asthma. Review. Cell Mol Life Sci. 2018; 75:3943–3961. Doi: 10.1007/s00018-018-2899-4.
  7. Paw M, Wnuk D, Kądziołka D, Sęk A, Lasota S, Czyż J, Madeja Z, Michalik M. Fenofibrate Reduces the Asthma-Related Fibroblast-To-Myofibroblast Transition by TGF- β /Smad2/3 Signaling Attenuation and Connexin 43-Dependent Phenotype Destabilization. Int J Mol Sci. 2018 Aug 29;19(9):2571. pii: E2571. doi: 10.3390/ijms19092571.
  8. Borowczyk-Michalowska J., Zimolag E., Konieczny P., Chrapusta A., Madeja Z., Drukala J. (2017) Stage Specific Embryonic Antigen-4 (SSEA-4) as a distinguishing marker between eccrine and apocrine origin of ducts of sweat glands. J Invest Dermatol 137(11):2437-2440. doi: 10.1016/j.jid.2017.07.003.
  9. Borowczyk-Michalowska J., Zimolag E., Waligorska A., Dobrucki J., Madeja Z., Drukala J. Stage-specific embryonic antigen-4 as a novel marker of ductal cells of human eccrine sweat glands. Br J Dermatol. 2017; 176(6): 1541-1548. DOI: 10.1111/bjd.15154.
  10. Piwowarczyk K, Paw M, Ryszawy D, Rutkowska-Zapała M, Madeja Z, Siedlar M, Czyż J. Connexin43high prostate cancer cells induce endothelial connexin43 up-regulation through the activation of intercellular ERK1/2-dependent signaling axis. Eur J Cell Biol. 2017 Jun;96(4):337-346.
  11. Paw M, Borek I, Wnuk D, Ryszawy D, Piwowarczyk K, Kmiotek K, Wójcik-Pszczoła KA, Pierzchalska M, Madeja Z, Sanak M, Błyszczuk P, Michalik M, Czyż J. Connexin43 Controls the Myofibroblastic Differentiation of Bronchial Fibroblasts from Patients with Asthma. Am J Respir Cell Mol Biol. 2017 Jul;57(1):100-110.
  12. Sroka J, Krecioch I, Zimolag E, Lasota S, Rak M, Kedracka-Krok S, Borowicz P, Gajek M, Madeja Z. Lamellipodia and membrane blebs drive efficient electrotactic migration of rat Walker carcinosarcoma cells WC 256. PLoS One. 2016;11(2):e0149133.
  13. Sarna M, Wójcik K, Hermanowicz P, Wnuk D, Burda K, Sanak M, Czyż J, Michalik M. Undifferentiated bronchial fibroblasts derived from asthmatic patients display higher elastic modulus than their non-asthmatic counterparts. PlosOne. 2015
  14. Labedz-Maslowska A, Lipert B, Berdecka D, Kedracka-Krok S, Jankowska U, Kamycka E, Sekula M, Madeja Z, Dawn B, Jura J, Zuba-Surma EK. Monocyte chemoattractant protein-induced protein 1 (MCPIP1) enhances angiogenic and cardiomyogenic potential of murine bone marrow-derived mesenchymal stem cells. PLoS One. 2015;10(7):e0133746.
  15. Piwowarczyk K, Wybieralska E, Baran J, Borowczyk J, Rybak P, Kosińska M, Włodarczyk AJ, Michalik M, Siedlar M, Madeja Z, Dobrucki J, Reiss K, Czyż J. Fenofibrate enhances barrier function of endothelial continuum within the metastatic niche of prostate cancer cells. Expert Opin Ther Targets. 2015;19(2):163-76.  
  16. Bobis-Wozowicz S, Kmiotek K, Sekula M, Kedracka-Krok S, Kamycka E, Adamiak M, Jankowska U, Madetko-Talowska A, Sarna M, Bik-Multanowski M, Kolcz J, Boruczkowski D, Madeja Z, Dawn B, Zuba-Surma EK. Human induced pluripotent stem cell-derived microvesicles transmit RNAs and proteins to recipient mature heart cells Modulating cell fate and behavior. Stem Cells. 2015;33(9):2748-61.
  17. Kozdęba M, Borowczyk J, Zimoląg E, Wasylewski M, Dziga D, Madeja Z, Drukala J. Microcystin-LR affects properties of human epidermal skin cells crucial for regenerative processes. Toxicon. 2014;80:38-46.
  18. Ryszawy D, Sarna M, Rak M, Szpak K, Kędracka-Krok S, Michalik M, Siedlar M, Zuba-Surma E, Burda K, Korohoda W, Madeja Z, Czyż J. Functional links between Snail-1 and Cx43 account for the recruitment of Cx43-positive cells into the invasive front of prostate cancer. Carcinogenesis. 2014;35(9):1920-30.
  19. Sroka J, Antosik A, Czyz J, Nalvarte I, Olsson JM, Spyrou G, Madeja Z. Overexpression of thioredoxin reductase 1 inhibits migration of HEK-293 cells. Biol Cell. 2007;99(12):677-87.

Batchelor/master thesis topics

  • Use of stem cells and progenitor cells in regenerative medicine (http://www.stemcells-project.eu/)
  • Role of stem cell derived extracellular vesicles (native and genetically modified) in tissue regeneration
  • Optimization of biocompatible scaffolds combining graphene and defined stem cell populations for tissue regeneration
  • Interaction between tumor cells and normal tissue: morphology, motility and cell-cell interaction between tumor cells and normal cells
  • Influence of cell-cell interactions on the activity of tumor cell migration
  • Modifying agents of cytoskeletal organization normal and neoplastic cells
  • Galvanotaxis and chemotaxis of various cell
  • Mechanisms of endocytosis
  • Intercellular communication through gap junctions
  • Mechanisms of cancer progression
  • Electrochemical methods in cell biology: cell separation, elec troperforation and electrofusion
  • The use of human cells cultured in vitro for transplantology
  • iPS- and MSC- derived extracellular vesicles as a promissing tool in in tissue regeneration
  • Impact of human extracellular vesicles derived from genetically modified stem cells on angigenic and cardiomyogenic potential of cardiac cells
  • Optimization of application of innovative biocompatible scaffolds and stem cells in tissue regeneration

Requirements for candidates

Basic knowledge on cell biology and molecular biology techniques – cell cultures and microscopy