Prof. dr hab. Maria Rąpała-Kozik
room: 4.0.26 (A103), phone: +48 12 664 65 27, e-mail: maria.rapala-kozik@uj.edu.pl
Dr. Dominika Bartnicka
room: 4.0.24 (A135), phone: +48 12 664 65 44, e-mail: dominika.ostrowska@uj.edu.pl
Dr. Grażyna Braś
room: 4.0.27 (A101), phone: +48 12 664 65 24, e-mail: grazyna.cholewa@uj.edu.pl
Dr. Justyna Karkowska-Kuleta
room: 4.0.27 (A101), phone: +48 12 664 65 24, e-mail: justyna.karkowska@uj.edu.pl
Dr. Dorota Satała
room: 4.0.27 (A101), phone: +48 12 664 65 24, e-mail: dorota.satala@uj.edu.pl
Dr. Marcin Zawrotniak
room: 4.0.24 (A135), phone: +48 12 664 65 44, e-mail: marcin.zawrotniak@uj.edu.pl
Mgr Magdalena Smolarz
Mgr Magdalena Surowiec
Mgr Miriam Gozalez-Gonzalez
- Yeast adhesion and invasion of the host cells
- Neutrophil Extracellular Traps (NETs) in fungal infections
- The role of aspartic proteases and adhesins of Candida spp. in fungal infections
- Moonlighting proteins at the surface of Candida spp. cells
- Interactions within the mixed biofilms involving bacterial and fungal opportunistic pathogens
- Biochemistry of vitamins and coenzymes
- The role of vitamin B1 (thiamine) in the protection of plant and yeast cells under stress conditions
- Gene expression analysis (QuantStudio 3 Real-Time PCR Systems)
- Recombinant protein production (E. coli, P. pastoris)
- Chromatographic techniques for separation and identification of biomolecules (FPLC, HPLC)
- Mass spectrometry coupled with liquid chromatography (LC-MS/MS) in proteomics
- Protein interaction analysis: surface plasmon resonance (SPR, BIACORE 3000), ELISA, fluorescence anisotropy, FRET
- Fluorescence microscopy (Olympus IX73)
- Maria Rapala-Kozik: Candida spp. fungi in aspiration pneumonia – accidental companions or environmental creators for bacterial anaerobial pathogen (2020–2023) OPUS 17, National Science Centre (NCN).
- Justyna Karkowska-Kuleta: Effect of fungal extracellular vesicles on the formation and stability of multispecies biofilms formed by pathogenic fungi from genus Candida (2019–2020). MINIATURA 3, National Science Centre (NCN).
- Marcin Zawrotniak: The role of extracellular neutrophilic DNA as an activating factor for the release of extracellular neutrophilic traps (2019–2020). MINIATURA 2, National Science Centre (NCN).
- Bartnicka, D., Gonzalez-Gonzalez, M., Sykut, J., Koziel, J., Ciaston, I., Adamowicz, K., Bras, G., Zawrotniak, M., Karkowska-Kuleta, J., Satala, D., Kozik, A., Zyla, E., Gawron, K., Lazarz-Bartyzel, K., Chomyszyn-Gajewska, M., & Rapala-Kozik, M. (2020). Candida albicans shields the periodontal killer Porphyromonas gingivalis from recognition by the host immune system and supports the bacterial infection of gingival tissue. International journal of molecular sciences, 21(6), 1984.
- Karkowska-Kuleta, J., Kulig, K., Karnas, E., Zuba-Surma, E., Woznicka, O., Pyza, E., Kuleta, P., Osyczka, A., Rapala-Kozik, M., & Kozik, A. (2020). Characteristics of extracellular vesicles released by the pathogenic yeast-like fungi Candida glabrata, Candida parapsilosis and Candida tropicalis. Cells, 9(7), 1722.
- Karkowska-Kuleta, J., Surowiec, M., Gogol, M., Koziel, J., Potempa, B., Potempa, J., Kozik, A., & Rapala-Kozik, M. (2020). Peptidylarginine deiminase of Porphyromonas gingivalis modulates the interactions between Candida albicans biofilm and human plasminogen and high-molecular-mass kininogen. International journal of molecular sciences, 21(7), 2495.
- Satala, D., Karkowska-Kuleta, J., Zelazna, A., Rapala-Kozik, M., & Kozik, A. (2020). Moonlighting proteins at the candidal cell surface. Microorganisms, 8(7), 1046.
- Guevara-Lora, I., Bras, G., Karkowska-Kuleta, J., González-González, M., Ceballos, K., Sidlo, W., & Rapala-Kozik, M. (2020). Plant-derived substances in the fight against infections caused by Candida Species. International journal of molecular sciences, 21(17), 6131.
- Zawrotniak, M., Wojtalik, K., & Rapala-Kozik, M. (2019). Farnesol, a quorum-sensing molecule of Candida albicans triggers the release of neutrophil extracellular traps. Cells, 8(12), 1611.
- Zawrotniak, M., Bartnicka, D., & Rapala-Kozik, M. (2019). UVA and UVB radiation induce the formation of neutrophil extracellular traps by human polymorphonuclear cells. Journal of photochemistry and photobiology. B, Biology, 196, 111511.
- Bartnicka, D., Karkowska-Kuleta, J., Zawrotniak, M., Satała, D., Michalik, K., Zielinska, G., Bochenska, O., Kozik, A., Ciaston, I., Koziel, J., Dutton, L. C., Nobbs, A. H., Potempa, B., Baster, Z., Rajfur, Z., Potempa, J., & Rapala-Kozik, M. (2019). Adhesive protein-mediated cross-talk between Candida albicans and Porphyromonas gingivalis in dual species biofilm protects the anaerobic bacterium in unfavorable oxic environment. Scientific reports, 9(1), 4376.
- Karkowska-Kuleta, J., Satala, D., Bochenska, O., Rapala-Kozik, M,, & Kozik, A. Moonlighting proteins are variably exposed at the cell surfaces of Candida glabrata, Candida parapsilosis and Candida tropicalis under certain growth conditions. BMC Microbiol. 2019 Jul 3;19(1):149.
- Karkowska-Kuleta, J., Bartnicka, D., Zawrotniak, M., Zielinska, G., Kieronska, A., Bochenska, O., Ciaston, I., Koziel, J., Potempa, J., Baster, Z., Rajfur, Z., & Rapala-Kozik, M. The activity of bacterial peptidylarginine deiminase is important during formation of dual-species biofilm by periodontal pathogen Porphyromonas gingivalis and opportunistic fungus Candida albicans. Pathog Dis. 2018 Jun 1;76(4):fty033
- Zawrotniak, M., Bochenska, O., Karkowska-Kuleta, J., Seweryn-Ozog, K., Aoki, W., Ueda, M., Kozik, A., & Rapala-Kozik, M. (2017). Aspartic Proteases and Major Cell Wall Components in Candida albicans Trigger the Release of Neutrophil Extracellular Traps. Frontiers in cellular and infection microbiology, 7, 414.
- The influence of Candida albicans virulence factors on the response of gingival fibroblasts
- The biological consequences of degradation of key host proteins by Candida spp. aspartic proteases
- Generation of Extracellular Traps (ET) by selected cells of the host immune system after contact with C. albicans cells and purified fungal virulence factors
- Changes in the gene expression of C. albicans selected virulence factors after contact with host cells
- Genetic and proteomic changes in the host defense cells in contact with C. albicans
- The formation of mixed biofilms by C. albicans and pathogenic bacteria – the analysis of factors involved in the cooperation between both types of microorganisms
- Identification of the protein-protein interactions of the major bacterial and fungal surface proteins, using SPR (surface plasmon resonance)
- Structural characteristics of thiamine biosynthetic enzymes using site directed mutagenesis.
Basic knowledge in biochemistry, molecular biology, cell biology, proteomics and instrumental analysis