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WBBiB

Wydział Biochemii, Biofizyki i Biotechnologii

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Department of General Biochemistry

 http://www.zbo.wbbib.uj.edu.pl

Prof. dr hab. Jolanta Jura 
room: B125 (3.0.29), phone: +48 12 664 63 59, e-mail: jolanta.jura@uj.edu.pl

Dr hab. Katarzyna Miękus
room: A116 (4.0.19), phone: +48 12 664 65 23, e-mail: katarzyna.miekus@uj.edu.pl

Dr Jerzy Kotlinowski
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: j.kotlinowski@uj.edu.pl

Dr Agata Lichawska-Cieślar
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: agata.lichawska@uj.edu.pl

Dr Mateusz Wilamowski
room: A145 (2.0.33), phone: +48 12 664 62 18, e-mail: mateusz.wilamowski@uj.edu.pl

Dr Jarosław Cisowski
room: A145 (2.0.33), phone: +48 12 664 62 18, e-mail: jaroslaw.cisowski@uj.edu.pl

Dr Tomasz Gromowski
room: A145 (2.0.33), phone: 12 664 62 18, e-mail: tomasz.gromowski@uj.edu.pl

Dr Judyta Górka
room: A115 (4.0.18), phone: 12 664 65 12, e-mail: judyta.gorka@uj.edu.pl

Dr Paulina Marona
room: A115 (4.0.18), phone: +48 12 664 65 12, e-mail: paulina.marona@uj.edu.pl

Mgr Monika Kolanek
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: monika.dziendziel@uj.edu.pl

Mgr  Karolina Podgórska 
room: A117 (4.0.20), phone: 12 664 65 31, e-mail: karolina1.podgorska@uj.edu.pl

 

Oliwia Kwapisz, room: A115 (4.0.18), phone: +48 12 664 65 12
Paweł Piłat, room: A115 (4.0.18), phone: +48 12 664 65 12
Weronika Szukała, room: A115 (4.0.18), phone: +48 12 664 65 12
Katarzyna Trzos, room: A115 (4.0.18), phone: +48 12 664 65 12
Piotr Czarnota, room: A115 (4.0.18), phone: + 48 12 664 65 12
Rafał Myrczek, room: A115 (4.0.18), phone: + 48 12 664 65 12
Szymon Surma, room: A115 (4.0.18), phone: + 48 12 664 65 12

  • Regulation of inflammatory processes
  • Activation of signaling pathways
  • Role of  MCPIP1 in adipogenesis
  • Structural analysis of MCPIP1
  • Molecular background of renal cell carcinoma: genetic and molecular studies
  • Cell differentiation, inflammation associated with skin disorders
  • Role of inflammation in etiology of metabolic diseases
  • miRNA/lncRNA/mRNA profile in selected disorders/pathogenic states

  • Culture of primary cells and cell lines
  • Gene transfer techniques: transfection, transduction
  • siRNA strategy
  • Reporter gene assays
  • PCR, real-time PCR, RACE, differential display
  • Western Blot, immunoprecipitation assay, immunohistochemical stainings
  • Molecular cloning – plasmid and viral vectors
  • Colorimetric, fluorimetric and luminometric tests: proliferation, migration, viability, apoptosis
  • In vivo models: tumor inoculation, conditional knockout mice, transgenic pig overexpressing MCPIP1 in adipose tissue

  1. Katarzyna Miękus: Molecular and cellular mechanisms responsible for the initiation and progression of clear cell renal cell carcinoma. (2023–2027). OPUS 23, NCN.
  2. Paulina Marona: The significance of Zc3h12a gene deletion in hepatocytes and proximal tubular epithelial cells for neoplastic transformation and sorafenib resistance. (2023–2027). OPUS 24, NCN.
  3. Agata Lichawska-Cieślar: Are MCPIP1 and MCPIP3 analogous or opposite players in the maintenance of skin function? (2023–2026). SONATA 18, NCN.
  4. Jerzy Kotlinowski: Role of Mcpip1 in biliary pathobiology: new potential therapeutic opportunities. (2022–2027). SONATA BIS 11, NCN
  5. Jolanta Jura: The role of PIN domain containing proteins in regulation of non-coding RNAs. (2022–2026). OPUS 21, NCN.
  6. Oliwia Kwapisz: Interplay between sex hormones and hepatocellular carcinoma progression in mice model. (2022–2025). PRELUDIUM 20, NCN
  7. Judyta Górka: Regulatory role of miRNAs and modification of β-catenin in the metastatic potential of clear cell renal cell carcinoma. (2022–2025). PRELUDIUM 20, NCN
  8. Jarosław Cisowski: Exploring the mechanisms of therapy-facilitated metastasis and CAAX proteins-processing enzymes in liver cancer. (2021–2025). OPUS 19, NCN.
  9. Weronika Szukała: MCPIP1 as a regulator of skin homeostasis. (2021–2024). PRELUDIUM 19, NCN.   
  10. Jerzy Kotlinowski: Analysis of MCPIP1 protein function in the etiology and development of primary cholangitis in mice – clinical implications. (2021–2023). Naukowa Fundacja Polpharmy.
  11. Katarzyna Miękus: Regulation of epithelial to mesenchymal transition and therapy resistance to antitumor therapies by MCPIP1. (2018–2023). SONATA BIS 7, NCN.

  1. Szukala W, Pilarczyk-Zurek M, Folkert J, Kotlinowski J, Koziel J, Jura J. Depletion of Mcpip1 in murine myeloid cells results in intestinal dysbiosis followed by allergic inflammation. Biochim Biophys Acta Mol Basis Dis. 2023; 1869(7):166764.
  2. Marona P, Górka J, Kwapisz O, Jura J, Rys J, Hoffman RM, Miekus K. Resistance to tyrosine kinase inhibitors promotes renal cancer progression through MCPIP1 tumor-suppressor downregulation and c-Met activation. Cell Death Dis. 2022; 13(9):814. 
  3. Kotlinowski J, Hutsch T, Czyzynska-Cichon I, Wadowska M, Pydyn N, Jasztal A, Kij A, Dobosz E, Lech M, Miekus K, Pośpiech E, Fu M, Jura J, Koziel J, Chlopicki S. Deletion of Mcpip1 in Mcpip1fl/flAlbCre mice recapitulates the phenotype of human primary biliary cholangitis. Biochim Biophys Acta Mol Basis Dis. 2021; 1867, 166086.
  4. Szukala W, Lichawska-Cieslar A, Pietrzycka R, Kulecka M, Rumienczyk I, Mikula M, Chlebicka I, Konieczny P, Dziedzic K, Szepietowski JC, Fontemaggi G, Rys J, Jura J. Loss of epidermal MCPIP1 is associated with aggressive squamous cell carcinoma. J Exp Clin Cancer Res. 2021; 40, 391.
  5. Gorka J, Marona P, Kwapisz O, Waligórska A, Pospiech E, Dobrucki JW, Rys J, Jura J, Miekus K. MCPIP1 inhibits Wnt/β-catenin signaling pathway activity and modulates epithelial-mesenchymal transition during clear cell renal cell carcinoma progression by targeting miRNAs. Oncogene. 2021 Oct 16. 
  6. Losko M, Dolicka D, Pydyn N, Jankowska U, Kedracka-Krok S, Kulecka M, Paziewska A, Mikula M, Major P, Winiarski M, Budzynski A, Jura J. Integrative genomics reveal a role for MCPIP1 in adipogenesis and adipocyte metabolism. Cell Mol Life Sci. 2020;77(23):4899-4919.
  7. Konieczny P, Lichawska-Cieslar A, Kwiecinska P, Cichy J, Pietrzycka R, Szukala W, Declercq W, Devos M, Paziewska A, Rumienczyk I, Kulecka M, Mikula M, Fu M, Borowczyk J, Santamaria-Babí LF, Jura J. Keratinocyte-specific ablation of Mcpip1 impairs skin integrity and promotes local and systemic inflammation. J Mol Med (Berl). 2019; 97, 1669-1684.
  8. Wilamowski M, Gorecki A, Dziedzicka-Wasylewska M, Jura J. Substrate specificity of human MCPIP1 endoribonuclease. Scientefic Reports. 2018. 8:7381 10.1038/s41598-018-25765-2
  9. Marona P, Górka J, Mazurek Z, Wilk W, Rys J, Majka M, Jura J, Miekus K. MCPIP1 Downregulation in Clear Cell Renal Cell Carcinoma Promotes Vascularization and Metastatic Progression. Cancer Res. 2017; Sep 15;77(18):4905-4920. 
  10. Ligeza J, Marona P, Gach N, Lipert B, Miekus K, Wilk W, Jaszczynski J, Stelmach A, Loboda A, Dulak J, Branicki W, Rys J, Jura J. MCPIP1 contributes to clear cell renal cell carcinomas development. Angiogenesis. 2017; Aug;20(3):325-340.

  • Expression alterations of chosen genes in regulation of inflammatory processes
  • Regulation alterations of mRNA stability, coding for cytokines and growth factors, in cancer, diabetes and metabolic syndrome pathogenesis 
  • MCPIP as an important regulator of cellular processes

Basic knowledge in biochemistry and molecular biology