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Department of General Biochemistry

WWW

 http://www.zbo.wbbib.uj.edu.pl

Head

prof. dr hab. Jolanta Jura (maiden name: Kwiatkowska)
room: B125 (3.0.29), phone: +48 12 664 63 59, e-mail: jolanta.jura@uj.edu.pl

Staff

dr Jerzy Kotlinowski
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: j.kotlinowski@uj.edu.pl

dr Katarzyna Miękus
room: A116 (4.0.19), phone: +48 12 664 65 23, e-mail: katarzyna.miekus@uj.edu.pl

dr Agata Lichawska-Cieślar
room: C144 (2.0.32), phone: +48 12 664 61 39, e-mail: agata.lichawska@uj.edu.pl

dr Dariusz Żurawek
room:  C145 (2.0.33), phone: +48 12 664 62 18, e-mail: dariusz.zurawek@uj.edu.pl

dr Tomasz Herjan
e-mail: tomasz.herjan@uj.edu.pl 

dr Mateusz Wilamowski
room: A115 (4.0.18), phone: +48 12 664 65 12 18, e-mail: mateusz.wilamowski@uj.edu.pl

mgr Monika Kolanek
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: monika.dziendziel@uj.edu.pl

mgr Monika Regiec
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: monika.hubacz@uj.edu.pl

mgr Barbara Strzelbicka
room: A117 (4.0.20), phone: +48 12 664 65 31, e-mail: barbara.strzelbicka@uj.edu.pl

PhD students

Oliwia Głąbica, room: A115 (4.0.18), phone: 12 664 65 12
Judyta Górka, room: A115 (4.0.18), phone: 12 664 65 12
Paulina Marona, room: A115 (4.0.18), phone: 12 664 65 12
Weronika Ostapczuk, room: A115 (4.0.18), phone: 12 664 65 12
Róża Pietrzycka, room: A115 (4.0.18), phone: 12 664 65 12
Natalia Pydyn, room: A115 (4.0.18), phone: 12 664 65 12

 

Research topics

  • Regulation of inflammatory processes
  • Activation of signaling pathways
  • Role of  MCPIP1 in adipogenesis
  • Structural analysis of MCPIP1
  • Molecular background of renal cell carcinoma: genetic and molecular studies
  • Cell differentiation, inflammation associated with skin disorders
  • Role of inflammation in etiology of metabolic diseases
  • miRNA/lncRNA/mRNA profile in selected disorders/pathogenic states

Methods

  • Culture of primary cells and cell lines
  • Gene transfer techniques: transfection, transduction
  • siRNA strategy
  • Reporter gene assays
  • PCR, real-time PCR, RACE, differential display
  • Western Blot, immunoprecipitation assay, immunohistochemical stainings
  • Molecular cloning – plasmid and viral vectors
  • Colorimetric, fluorimetric and luminometric tests: proliferation, migration, viability, apoptosis
  • In vivo models: tumor inoculation, conditional knockout mice, transgenic pig overexpressing MCPIP1 in adipose tissue

Current projects

  1. Jolanta Jura: Role of MCPIP1 protein in epidermis physiology and pathophysiology.  (2017-2020). OPUS 12, National Science Centre (NCN).
  2. Tomasz Herjan: The function of the RNA-binding protein HuR in IL-17-associated severe astma. (2017-2020). SONATA 11, National Science Centre (NCN).
  3. Jerzy Kotlinowski: MCPIP1 as an important regulator of development and progression of non-alcoholic fatty liver disease. (2016-2020). SONATA 10, National Science Centre (NCN).
  4. Jerzy Kotlinowski: MCPIP1 as an important regulator of development and progression of nonalcoholic fatty liver disease. (2018-2021). OPUS 14, National Science Centre (NCN).
  5. Katarzyna Miękus: The role of MCPIP1 protein in growth, progression and vascularisation of clear cell renal cell carcinoma. (2016-2020). OPUS 10, National Science Centre (NCN).
  6. Katarzyna Miękus: Regulation of epithelial-mesenchymal transition and resistance to anti-cancer therapies used by the MCPIP1 protein. (2018-2023). SONATA BIS 7, National Science Centre (NCN).
  7. Paulina Marona: The role of MCPIP1 as a potential suppressor in the development and therapy of clear cell carcinoma renal (2018-2020). PRELUDIUM 13, National Science Centre (NCN).

Selected publications

  1. Kotlinowski J, Bukowska-Strakova K, Koppolu A, Kosinska J, Pydyn N, Stawinski P, Wilamowski M, Nowak W, Jozkowicz A, Baran J, Ploski R, Jura J. A Novel Monoallelic Nonsense Mutation in the NFKB2 Gene Does Not Cause a Clinical Manifestation. Front. Genet. 2019; Feb 26 10:140. doi: 10.3389/fgene.2019.00140
  2. Wilamowski M, Gorecki A, Dziedzicka-Wasylewska M, Jura J. Substrate specificity of human MCPIP1 endoribonuclease. Scientefic Reports. 2018. 8:7381 10.1038/s41598-018-25765-2
  3. Lichawska- Cieslar A, Pietrzycka R, Ligeza J, Kulecka M, Paziewska A, Kalita A, Dolicka D.D, Wilamowska M, Miekus K, Ostrowski J, Mikula M, Jura J. RNA sequencing reveals widespread transcriptome changes in a renal carcinoma cell line. Oncotarget. 2018; Vol. 9, (No. 9), pp: 8597-8613.
  4. Losko M, Lichawska-Cieslar A, Kulecka M, Paziewska A, Rumienczyk I, Mikula M, Jura J. Ectopic overexpression of MCPIP1 impairs adipogenesis by modulating microRNAs. Biochim Biophys Acta. 2017; Sep 19. pii: S0167-4889(17)30253-7. 
  5. Marona P, Górka J, Mazurek Z, Wilk W, Rys J, Majka M, Jura J, Miekus K. MCPIP1 Downregulation in Clear Cell Renal Cell Carcinoma Promotes Vascularization and Metastatic Progression. Cancer Res. 2017; Sep 15;77(18):4905-4920. 
  6. Lipert B, Wilamowski M, Gorecki A, Jura J. MCPIP1, alias Regnase-1 binds and cleaves mRNA of C/EBPβ. PLoS One. 2017; Mar 22;12(3):e0174381. 
  7. Ligeza J, Marona P, Gach N, Lipert B, Miekus K, Wilk W, Jaszczynski J, Stelmach A, Loboda A, Dulak J, Branicki W, Rys J, Jura J. MCPIP1 contributes to clear cell renal cell carcinomas development. Angiogenesis. 2017; Aug;20(3):325-340. 
  8. Dobosz E, Wilamowski M, Lech M, Bugara B, Jura J, Potempa J, Koziel J. MCPIP-1, alias Regnase-1 controls epithelial inflammation by post-transcriptional regulation of IL-8 production. J Innate Immun. 2017. 8(6): 564–578. doi:10.1159/000448038.
  9. Ruiz-Romeu E, Ferran M, Giménez-Arnau A, Bugara B, Lipert B, Jura J, Florencia EF, Prens EP, Celada A, Pujol RM, Santamaria-Babi LF. MCPIP1 RNase is aberrantly distributed in psoriatic epidermis and rapidly induced by IL-17A. J Invest Dermatol. 2016; S0022-202X(16)31153-8.
  10. Skrzypek K, Kusienicka A, Szewczyk B, Adamus T, Lukasiewicz E, Miekus K, Majka M. Constitutive activation of MET signaling impairs myogenic differentiation of rhabdomyosarcoma and promotes its development and progression. Oncotarget. 2015;13;6(31):31378-98.

 

Batchelor/master thesis topics

  • Expression alterations of chosen genes in regulation of inflammatory processes
  • Regulation alterations of mRNA stability, coding for cytokines and growth factors, in cancer, diabetes and metabolic syndrome pathogenesis  
  • MCPIP as an important regulator of inflammatory state
  • Role of protein kinases in mitogenic signal transmission
  • Nutrients impact on level of endogenous oxidative DNA damage

Requirements for candidates

Basic knowledge in biochemistry and molecular biology