Prof. dr hab. Joanna Bereta
room: B122 (3.0.26), phone: +48 12 664 63 56, e-mail: joanna.bereta@uj.edu.pl

Dr hab. Aneta Kasza
room: A114 (4.0.17), phone: +48 12 664 65 21, e-mail: aneta.kasza@uj.edu.pl

Dr hab. Monika Bzowska
room: B123 (3.0.27), phone: +48 12 664 63 88, e-mail: monika.bzowska@uj.edu.pl

Dr Alicja Hinz
room: B123 (3.0.27), phone: +48 12 664 63 88, e-mail: alicja.hinz@uj.edu.pl  

Dr Jakub Kochan
room: C142 (2.0.34), phone: +48 12 664 62 13, e-mail: jakub.kochan@uj.edu.pl  

Dr Renata Mężyk-Kopeć
room: B118 (3.0.22), phone: +48 12 664 63 41, e-mail: renata.mezyk-kopec@uj.edu.pl

Dr Krystyna Stalińska
room: B119 (3.0.23), phone: +48 12 664 63 99, e-mail: krystyna.stalinska@uj.edu.pl

Dr Mateusz Wawro
room: C142 (2.0.34), phone: +48 12 664 62 13, e-mail: mateusz.wawro@uj.edu.pl 

Mgr Urszula Tuleja
room: B120 (3.0.24), phone: +48 12 664 63 57, e-mail: urszula.tuleja@uj.edu.pl

Kornelia Kłosińska
room: C138 (2.0.14), phone: +48 12 664 61 41, e-mail: kornelia.klosinska@uj.edu.pl

Joanna Król, room: B118 (3.0.22), phone: +48 12 664 63 41, e-mail: joanna.krol@doctoral.uj.edu.pl

Aleksandra Solecka, room: C142 (2.0.34), phone: +48 12 664 64 00, e-mail: aleksandra.solecka@doctoral.uj.edu.pl

Weronika Sowińska, room: C142 (2.0.34), phone: +48 12 664 64 00, e-mail: weronika.sowinska@doctoral.uj.edu.p

• Regulation of the expression of genes involved in inflammation and cancer
• Influence of cytokines and growth factors on the expression of selected genes
• Biological evaluation of nanomaterials and biomaterials 
• Mechanisms involved in the regulation of transcript stability
• Generation and characterization of monoclonal antibodies specific to selected proteins – their applications in research, diagnostics and therapies
• Influence of hyperthermia on repair of DNA damage in tumor cells
• Structure, stability, and functions of mouse IgG3 immunoglobulins in the light of their natural roles and potential use in diagnostics and therapies
• Functions, regulation of expression, and the mechanism of action of sheddases of the ADAM family

• Methods of molecular biology: PCR, RT-PCR, qRT-PCR, EMSA, restriction analysis, genetic engineering, generation of genetic constructs, directed mutagenesis, gene silencing with siRNA and and CRISPRi, transient and stable transfection of eukaryotic cells
• Genome editing tools: CRISPR/CAS9 and TALEN
• Protein expression in prokaryotic and eukaryotic systems
• Generation of monoclonal antibodies using cell fusion (hybridoma)- and phage display methodologies
• Modifications of proteins including antibodies
• Immunochemical techniques: ELISA, Western blotting, flow cytometry
• Immunofluorescence microscopy combined with RNA FISH 
  Analysis of enzyme activities (e.g. proteases, SOD, iNOS, LDH, other oxidoreductases)
  Microbiological methods, e.g. analysis of infectivity and cytotoxic potential of Salmonella strains 
  Analysis of cell metabolism in cell cultures
  In vivo studies of nanoparticle-based drug delivery and cancer treatments in mouse models

1. Aneta Kasza: The role of RNase ZC3H12B in glioblastoma multiforme pathophysiology. (2021–2025). National Science Centre (NCN).
2. Jakub Kochan: The NORAD-stress granule axis and its role in the hyperthermia-induced radio-/chemosensitization of cancer cells. (2021–2024). National Science Centre (NCN).

1. Fluorophore Localization Determines the Results of Biodistribution of Core-Shell Nanocarriers. Hinz A, Szczęch M, Szczepanowicz K, Bzowska M. Int J Nanomedicine. 2022, 17:577-588. 
2. Addressing the Osteoporosis Problem — Multifunctional Injectable Hybrid Materials for Controlling Local Bone Tissue Remodeling Gilarska A, Hinz A, Bzowska M, Dyduch G, Kamiński K, Nowakowska M and Lewandowska-Łańcucka J. ACS Appl. Mater. Interfaces 2021, 13, 42, 49762-49779.
3. Non-targeting control for MISSION shRNA library silences SNRPD3 leading to cell death or permanent growth arrest. Czarnek M, Sarad K, Karaś A, Kochan J, Bereta J. Mol Ther Nucleic Acids 2021, 26: 711–731
4. Molecular Mechanisms of ZC3H12C/Reg-3 Biological Activity and Its Involvement in Psoriasis Pathology. Wawro M, Kochan J, Sowinska W, Solecka A, Wawro K, Morytko A, Kwiecinska P, Grygier B, Kwitniewski M, Fu M, Cichy J, Kasza A. Int J Mol Sci. 2021, 22:7311. 
5. Proteolytic Processing of Neuregulin 2. Czarnek M, Bereta J. Mol Neurobiol. 2020 Apr;57(4):1799-1813.
6. In vivo Studies on Pharmacokinetics, Toxicity and Immunogenicity of Polyelectrolyte Nanocapsules Functionalized with Two Different Polymers: Poly-L-Glutamic Acid or PEG. Karabasz A, Szczepanowicz K, Cierniak A, Mezyk-Kopec R, Dyduch G, Szczęch M, Bereta J, Bzowska M. Int J Nanomed. 2019 Dec 5;14:9587-9602. 
7. Potential limitations of the Sleeping Beauty transposon use in gene expression studies. Sowińska W, Wawro M, Solecka A, Kasza A. Acta Biochim Pol. 2019 Jul 12;66(3):263-268. 
8. ZC3H12B/MCPIP2, a new active member of the ZC3H12 family Wawro M, Wawro K, Kochan J, Solecka A, Sowinska W, Lichawska-Cieslar A, Jura J, Kasza A. RNA. 2019 Jul; 25(7):840-856. 
9. Ultra-soft X-ray system for imaging the early cellular responses to X-ray induced DNA damage. Kochan JA, van den Belt M, von der Lippe J, Desclos ECB, Steurer B, Hoebe RA, Scutigliani EM, Verhoeven J, Stap J, Bosch R, Rijpkema M, van Oven C, van Veen HA, Stellingwerf I, Vriend LEM, Marteijn JA, Aten JA, Krawczyk PM. Nucleic Acids Res. 2019 Sep 26;47(17):e100.
10. CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination. Klaus T, Bereta J. Front Immunol. 2018 May 23;9:1096.

The topics for B.Sc. and M.Sc. thesis are directly related to the current research lines that are pursued at our Department.

The students are expected to have a sound background in biochemistry and molecular biology, as well as good language skills in English.