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Department of Cell Biochemistry

Head

Prof. Joanna Bereta, PhD, DSc
room: B122 (3.0.26), phone: +48 12 664 63 56, e-mail: joanna.bereta@uj.edu.pl

Staff

Dr hab. Aneta Kasza
room: A114 (4.0.17), phone: +48 12 664 65 21, e-mail: aneta.kasza@uj.edu.pl

Dr. Monika Bzowska
room: B123 (3.0.27), phone: +48 12 664 63 88, e-mail: monika.bzowska@uj.edu.pl

Dr. Krystyna Stalińska
room: B119 (3.0.23), phone: +48 12 664 63 99, e-mail: krystyna.stalinska@uj.edu.pl

Dr. Renata Mężyk-Kopeć
room:  B118 (3.0.22), phone: +48 12 664 63 38, e-mail: renata.mezyk-kopec@uj.edu.pl

Dr. Mateusz Wawro
room: C142 (2.0.34), phone: +48 12 664 64 00, e-mail: mateusz.wawro@uj.edu.pl 

Mgr Alicja Karabasz
room: B123 (3.0.27), phone: +48 12 664 63 88, e-mail:  alicja.karabasz@doctoral.uj.edu.pl 

Mgr Aleksandra Solecka
room: C142 (2.0.34), phone: +48 12 664 64 00

Mgr Weronika Sowińska
room: C142 (2.0.34), phone: +48 12 664 64 00

Mgr Urszula Tuleja
room: B120 (3.0.24), phone: +48 12 664 63 57, e-mail: urszula.tuleja@uj.edu.pl

 

PhD students

Maria Czarnek, room: B123 (3.0.27), phone: +48 12 664 63 88

Alicja Karabasz, room: B129 (3.0.21), phone: +48 12 664 64 04

Aleksandra Solecka, room: C142 (2.0.34), phone: +48 12 664 64 00

Weronika Sowińska, room: C142 (2.0.34), phone: +48 12 664 64 00

Edyta Żyła, Małopolska Center of Biotechnology

Research topics

  • Regulation of the expression of genes involved in inflammation and cancer
  • Influence of cytokines and growth factors on the expression of selected genes
  • Functions, regulation of expression, and the mechanism of action of sheddases of the ADAM family
  • Mechanisms involved in the regulation of transcript stability
  • Generation and characterization of monoclonal antibodies specific to selected proteins – their applications in research, diagnostics and therapies
  • Influence of hyperthermia on repair of DNA damage in tumor cells
  • Structure, stability, and functions of mouse IgG3 immunoglobulins in the light of their natural roles and potential use in diagnostics and therapies
  • Nanoparticle-based drug delivery systems
  • Genetic modifications of Salmonella enterica Typhimurium strains for the improvement of their antitumor potentia

Methods and specialized equipment

  • Methods of molecular biology: PCR, RT-PCR, qRT-PCR, EMSA, restriction analysis, genetic engineering, generation of genetic constructs, directed mutagenesis, gene silencing with siRNA, transient and stable transfection of eukaryotic cells
  • Genome editing tools: CRISPR/CAS9
  • Protein expression in prokaryotic and eukaryotic systems
  • Generation of monoclonal antibodies using cell fusion (hybridoma)- and phage display methodologies
  • Modifications of proteins including antibodies
  • Immunochemical techniques: ELISA, Western blotting, flow cytometry
  • Immunofluorescence microscopy combined with RNA FISH 
    Analysis of enzyme activities (e.g. proteases, SOD, iNOS, LDH, other oxidoreductases)
    Microbiological methods, e.g. analysis of infectivity and cytotoxic potential of Salmonella strains 
    Analysis of cell metabolism in cell cultures
    In vivo studies of nanoparticle-based drug delivery and cancer treatments in mouse models

Current projects

  1. Joanna Bereta: ADAM17 as a key protein in cellular response to proinflammatory mediators (2017-2020). OPUS 11, National Science Centre (NCN).
  2. Aneta Kasza: PAnalysis of the inflammatory role of MCPIP2 using the CRISPR/dCas9-FokI system. Identification of MCPIP2 substrates and interacting proteins, as well as factors controlling MCPIP2 expression. (2015-2019). National Science Centre (NCN).

Selected publications

  1. ZC3H12B/MCPIP2, a new active member of the ZC3H12 family Wawro M, Wawro K, Kochan J, Solecka A, Sowinska W, Lichawska-Cieslar A, JuraS J, Kasza A. RNA. 2019 Jul; 25(7):840-856. 
  2. Ultra-soft X-ray system for imaging the early cellular responses to X-ray induced DNA damage. Kochan JA, van den Belt M, von der Lippe J, Desclos ECB, Steurer B, Hoebe RA, Scutigliani EM, Verhoeven J, Stap J, Bosch R, Rijpkema M, van Oven C, van Veen HA, Stellingwerf I, Vriend LEM, Marteijn JA, Aten JA, Krawczyk PM. Nucleic Acids Res. 2019 Sep 26;47(17):e100.
  3. Analysis of toxicity and anticancer activity of micelles of sodium alginate-curcumin.Karabasz A, Lachowicz D, Karewicz A, Mezyk-Kopec R, Stalińska K, Werner E, Cierniak A, Dyduch G, Bereta J, Bzowska M. Int J Nanomedicine. 2019 Sep6;14:7249-7262.
  4. CH2 Domain of Mouse IgG3 Governs Antibody Oligomerization, Increases Functional Affinity to Multivalent Antigens and Enhances Hemagglutination. Klaus T, Bereta J. Front Immunol. 2018 May 23;9:1096.
  5. Mouse Antibody of IgM Class is Prone to Non-Enzymatic Cleavage between CH1 and CH2 Domains. Klaus T, Stalińska K, Czaplicki D, Mak P, Skupien-Rabian B, Kedracka-Krok S, Wiatrowska K, Bzowska M, Machula M, Bereta J. Sci Rep. 2018 Jan 11;8(1):519.
  6. In vitro toxicity studies of biodegradable, polyelectrolyte nanocapsules. Karabasz A, Szczepanowicz K, Cierniak A, Bereta J, Bzowska M. Int J. Nanomedicine. 2018 Sep 6;13:5159-5172.
  7. SmartFlares fail to reflect their target transcripts levels. Czarnek M, Bereta J. Sci Rep. 2017 Sep 15;7(1):11682.
  8. Intact NYN/PIN-Like Domain is Crucial for the Degradation of Inflammation-Related Transcripts by ZC3H12D. Wawro M, Kochan J, Krzanik S, Jura J, Kasza A. J Cell Biochem. 2017 Mar;118(3):487-498.
  9. The CRISPR-Cas system - from bacterial immunity to genome engineering. Postepy Hig Med Dosw (Online). Czarnek M, Bereta J. 2016 Sep 1;70(0):901-16. Review.10. IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA. Kochan J, Wawro M, Kasza A. Biol Open. 2016 Jun 2. pii: bio.018010.
  10. IF-combined smRNA FISH reveals interaction of MCPIP1 protein with IER3 mRNA. Kochan J, Wawro M, Kasza A. Biol Open. 2016 Jun 2. pii: bio.018010.

Batchelor/master thesis topics

The topics for B.Sc. and M.Sc. thesis are directly related to the current research lines that are pursued at our Department.

Requirements for candidates

The students are expected to have a sound background in biochemistry and molecular biology, as well as good language skills in English.