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Department of Physical Biochemistry

Webpage

http://www.zbf.wbbib.uj.edu.pl

Head

Prof. dr hab. Marta Dziedzicka–Wasylewska
room: C021 (2.01.22), phone: +48 12 664 61 22, e-mail: marta.dziedzicka-wasylewska@uj.edu.pl

Staff

Dr hab. Sylwia Kędracka-Krok
proom: C020 (2.01.21), phone: +48 12 664 61 48, e-mail: sylwia.kedracka-krok@uj.edu.pl

Dr hab. Andrzej Górecki
room: C023 (2.01.24), phone: +48 12 664 61 51, e-mail: andrzej.gorecki@uj.edu.pl

Dr hab. Sylwia Łukasiewicz
room: C022 (2.01.23), phone: +48 12 664 61 34, e-mail: sylwia.lukasiewicz@uj.edu.pl

Dr Piotr Bonarek
room: C023 (2.01.24), phone: +48 12 664 61 51, e-mail: piotr.bonarek@uj.edu.pl

Dr Agnieszka Polit
room: C019 (2.01.20), phone: +48 12 664 61 56, e-mail: a.polit@uj.edu.pl

Dr Ewa Błasiak
room: C019 (2.01.20), phone: +48 12 664 61 56, e-mail: ewa.blasiak@uj.edu.pl

Dr Ewelina Fic
room: C022 (2.01.23), phone: +48 12 664 61 34, e-mail: ewelina.fic@uj.edu.pl

Dr Małgorzata Figiel
room: C022 (2.01.23), phone: +48 12 664 61 34, e-mail: m.figiel@uj.edu.pl

Dr Paweł Mystek
room: C024 (2.01.25), phone: +48 12 664 61 54, e-mail: pawel.mystek@uj.edu.pl

PhD students

Mgr Beata Rysiewicz, room: C024 (2.01.25), phone: +48 12 664 61 54
Mgr Julia Łakomska, room: C025 (2.01.26), phone: + 48 12 664 61 55
Mgr Dorota Mularczyk, room: C025 (2.01.26), phone: + 48 12 664 61 55
Mgr Agata Kowalik, room: C025 (2.01.26), phone: + 48 12 664 61 55

Research topics

  • Molecular mechanisms of eukariotic genes transcription activation by Yin Yang 2 (YY2)
  • Interaction of YY1 and YY1 mutants with molecular partners and DNA promoter sequences
  • Ligand binding specificity of beta lactoglobulin
  • Mechanism of selected drugs binding by beta lactoglobulin
  • Mechanisms of GPCRs and G proteins interactions
  • Mechanisms of GPCRs and G proteins interactions with lipid membranes
  • Efficient production and purification of GPCRs
  • Role of homo- and hetero oligomerization of GPCRs
  • Nanoparticles selectively recognizing GPCR oligomers
  • Influence of antidepressants and antipsychotic drugs on proteome
  • Proteomic characterization of T cells subpopulations isolated from blood of depressive  patients

Methods and specialized equipment

  • Molecular biology techniques: cloning, mutagenesis
  • Recombinant protein production and purification: New Brunswick Innova 43R, Amersham, ÄktaExplorer
  • Confocal microscopy (FRET-FLIM, FRAP)
  • Isothermal and differential microcalorimetry (ITC, DSC): MicroCal, CSC 6100
  • Spectroscopic methods (circular dichroism, steady-state and time resolved fluorescence, fluorescence anisotropy, FRET): Jasco J-170, Horiba, system 5000U
  • Fast kinetics techniques (T-jump, stopped-flow): Applied Photophysics SX-17 MW, Hi-Tech Scientific PTJ-64
  • Two-dimensional electrophoresis (2DE): GE Healthcare Ettan DALTsix
  • Differential in gel electrophoresis (DIGE)
  • Mass spectrometry coupled with liquid chromatography (LC-MS/MS) in proteomics

Current projects

  1. Agnieszka Polit: The effect of membrane domains on the G protein-lipid interaction (2017-2020). OPUS 12, National Science Centre (NCN).
  2. Sylwia Kędracka-Krok: Characteristics of clozapine and risperidone at the level of the cell nucleus (2018-2021). OPUS 13, National Science Centre (NCN).

Selected publications

  1. Figiel M, Łakomska J, Miłek P, Dziedzicka-Wasylewska M, Górecki A. The transcription factor YY2 has less momentous properties of an intrinsically disordered protein than its paralog YY1. FEBS Lett. 2019 Jul;593(14):1787-1798.
  2. Bonarek P, Polit A. Systematic calorimetric studies of proton exchange associated with binding of beta-lactoglobulin with ligand. Int J Biol Macromol2018 Dec;120(Pt A):128-134.
  3. Kedracka-Krok S, Swiderska B, Bielecka-Wajdman AM, Prus G, Skupien-Rabian B, Jankowska U, Obuchowicz E. Impact of imipramine on proteome of rat primary glial cells. J Neuroimmunol2018 Jul 15;320:25-37.
  4. Górka AK, Górecki A, Dziedzicka-Wasylewska M. Site-directed fluorescence labeling of intrinsically disordered region of human transcription factor YY1: The inhibitory effect of zinc ions. Protein Sci2018 Feb;27(2):390-401.
  5. Łukasiewicz S, Szczepanowicz K, Podgórna K, Błasiak E, Majeed N, Ogren SO, Nowak W, Warszyński P, Dziedzicka-Wasylewska M. Encapsulation of clozapine in polymeric nanocapsules and its biological effects. Colloids Surf B Biointerfaces2016;140:342-52.
  6. Górecki A, Bonarek P, Górka AK, Figiel M, Wilamowski M, Dziedzicka-Wasylewska M. Intrinsic disorder of human Yin Yang 1 protein. Proteins2015;83(7):1284-96.
  7. Mystek P, Tworzydło M, Dziedzicka-Wasylewska M, Polit A. New insights into the model of dopamine D1 receptor and G-proteins interactions. Biochim Biophys Acta2015 ;1853(3):594-603.
  8. Kędracka-Krok S, Swiderska B, Jankowska U, Skupień-Rabian B, Solich J, Buczak K, Dziedzicka-Wasylewska M. Clozapine influences cytoskeleton structure and calcium homeostasis in rat cerebral cortex and has a different proteomic profile than risperidone. J Neurochem2015;132(6):657-76.
  9. Lukasiewicz S, Polit A, Kędracka-Krok S, Wędzony K, Mackowiak M, Dziedzicka-Wasylewska M. Hetero-dimerization of serotonin 5-HT(2A) and dopamine D(2) receptors. Biochim Biophys Acta2010 Dec;1803(12):1347-58
  10. Fic E, Kedracka-Krok S, Jankowska U, Pirog A, Dziedzicka-Wasylewska M. Comparison of protein precipitation methods for various rat brain structures prior to proteomic analysis. Electrophoresis2010 Oct;31(21):3573-9.

Requirements for candidates

  • Basic knowledge on structural biochemistry
  • Basic knowledge on molecular biology and  protein engineering
  • Basic knowledge on protein production and purification
  • Basic knowledge on proteomics