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WBBiB

Wydział Biochemii, Biofizyki i Biotechnologii

Laboratory of Stem Cells Biology

https://www.nicheworks.eu/hscs-and-leukemia

Dr Krzysztof Szade
room: D205 (1.1.0)
phone: +48 12 664 60 24
e-mail: krzysztof.szade@uj.edu.pl

Dr Natalia Bryniarska-Kubiak, adiunkt
room: 1.01.4, phone: +48 12 664 60 25, e-mail: natalia.bryniarska@uj.edu.pl

Dr Andrzej Kubiak 
room: 1.01.4, phone: +48 12 664 60 25, e-mail: andrzej.kubiak@uj.edu.pl

Dr Patrycja Kwiecińska
room: 1.01.4, phone: +48 12 664 60 25, e-mail: patrycja.kwiecinska@uj.edu.pl

Mgr Kacper Kowalski
room: 3.01.7, phone: +48 12 664 63 98, e-mail: kacper1.kowalski@uj.edu.pl

Izabella Skulimowska, room 3.01.7, phone: + 48 12 664 63 98, e-mail: izabella.skulimowska@uj.edu.pl

Jadwiga Filipek-Gorzała, room 1.01.4: phone: +48 12 664 60 25, e-mail: j.filipek-gorzala@doctoral.uj.edu.pl

  • Self-renewal and differentiation of hematopoietic stem cells
  • Development of blood malignancies 
  • Role of adult stem cells in tissue regeneration 

Instruments
  • 10x platform for single cell RNA sequencing  
  • MissionBio Tapestri for targeted DNA sequencing in single cells 
Methods
  • Multiparameter flow cytometry i cel sorting 
  • NGS transcriptome and genome analysis 
  • PDX models of blood malignancies in immunocompromised mice 
  • Bone-marrow transplantation models 
  • Functional clonal analysis of stem cells 

  1. Andrzej Kubiak: The role of mechanical interactions in the chondrogenic differentiation of stromal cells (2023–2024), Miniatura, NCN
  2. Jadwiga Filipek-Gorzała: Epigenetic memory of hem. (2023–2024), Research Support Module for PhD students within IDUJ programme.
  3. Krzysztof Szade: What does your blood remember? The memory of hematopoietic stem cells. (2022–2027), ERC Starting Grant
  4. Krzysztof Szade :Identifying the hematopoietic and leukemic stem cell niches. (2021–2023), Mozart, NCN
  5. Krzysztof Szade: Tracing the roots of leukemia – how pre-leukemic hematopoietic stem cells contribute to acute lymphoid and myeloid leukemias? (2018–2022), Harmonia, NCN

  1. Gulati GS, Zukowska M, Noh JJ, Zhang A, Wesche DJ, Sinha R, George BM, Weissman IL, Szade K. Neogenin-1 distinguishes between myeloid-biased and balanced Hoxb5 + mouse long-term hematopoietic stem cells. PNAS 2019 Dec 10;116(50):25115-25125. doi: 10.1073/pnas.1911024116
  2. Szade K, Zukowska M, Szade A, Nowak W, Skulimowska I, Ciesla M, Bukowska-Strakova K, Gulati GS, Kachamakova-Trojanowska N, Kusienicka A, Einwallner E, Kijowski J, Czauderna S, Esterbauer H Benes V, L Weissman I, Dulak J, Jozkowicz A. Heme oxygenase-1 deficiency triggers exhaustion of hematopoietic stem cells. EMBO Rep. 2020 Feb 5;21(2):e47895. doi: 10.15252/embr.201947895
  3. Skulimowska I, Sosniak J, Gonka M, Szade A, Jozkowicz A, Szade K. The biology of hematopoietic stem cells and its clinical implications. FEBS J. 2021 Sep 8. doi: 10.1111/febs.16192. 

  • Mechanism of self-renwal and differentiation of hematopoietic stem cells 
  • Preleukemic hematopoietic stem cells
  • Clonal and lineage tracing during hematopoiesis 
  • Machine learning for analysis of single stem cells differentiation 

Basic methods of molecular and cellular biology