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WBBiB

Wydział Biochemii, Biofizyki i Biotechnologii

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Laboratory of Molecular Genetics and Virology

Dr​ hab. Irena Horwacik
room: B139 (3.0.32), phone: +48 12 664 63 69, e-mail: irena.horwacik@uj.edu.pl

Prof. dr hab. Hanna Rokita
room: B140 (3.0.31), phone: +48 12 664 63 37, e-mail: hanna.rokita@uj.edu.pl

Dr inż. Beata Bakera
room. B101 (3.0.36), phone: +48 12 664 6376, e-mail: beata.bakera@uj.edu.pl

Dr Małgorzata Durbas
room: B141 (3.0.30), phone: +48 12 664 63 60, e-mail: malgorzata.durbas@uj.edu.pl

Mgr inż. Anna Gontarska
room: B141 (3.0.30), phone: +48 12 664 63 60

Maja Kudrycka, room: B101 (3.0.36), phone: +48 12 664 63 76

Agnieszka Ostromęcka, room: B101 (3.0.36), phone: +48 12 664 6376, e-mail: agnieszka.ostromecka@uj.edu.pl

  • Chemoimmunotherapy of cancer of neuroectodermal origin
  • Molecular mechanisms of neuroblastoma cell death induced by monoclonal antibodies binding to GD2 ganglioside

Methods

  • DNA microarrays and proteomic arrays
  • Quantitative RT-PCR
  • Western blotting
  • Flow cytometry
  • Fluoresence microscopy
  • Gene overexpression and gene silencing 

Equipment

  • Apparatus for qRT-PCR, Illumina

 

  1. Hanna Rokita: Contribution of PHLDA1 to response of neuroblastoma cells to clinical and experimental treatment of high-risk neuroblastoma. (2019–2023). OPUS 15, 2018/29/B/NZ7/01564, National Science Center

  1. Durbas M, Rokita H, Horwacik I, Wiśniewska A, Nowak I. (2022) Apoptosis is responsible for the cytotoxic effects of anti-GD2 ganglioside antibodies and aurora A kinase inhibitors on human neuroblastoma cells. Acta Biochim. Pol. 69:485-494. doi: 10.18388/abp.2020_634.
  2. Nowak I, Brożyna AA, Zabłocka M, Student S, Durbas M, Bugara B, Rokita H. (2021) MCPIP1 expression positively correlates with melanoma-specific survival of patients, and its overexpression affects vital intracellular pathways of human melanoma cells. Mol. Carcinog. 60:227-241. Doi.org/10.1002/mc.23286.
  3. Nowak I, Boratyn E, Student S, Bernhart SF, Fallmann J, Durbas M, Stadler PF, Rokita H. (2021) MCPIP1 ribonuclease can bind and cleave AURKA mRNA in MYCN-amplified neuroblastoma cells. RNA Biol. 18:144-156.  Doi.org/10.1080/15476286.2020.1804698.
  4. Boratyn E, Nowak I, Karnas E, Ryszawy D, Wnuk D, Polus A, Durbas M, Horwacik I, Rokita H. (2020) MCPIP1 overexpresion in human neuroblastoma cell lines causes cell-cycle arrest by G1/S checkpoint block. J. Cell. Biochem. 121:3406-3425. Doi: 10.1002/jcb.29614.
  5. Durbas M, Pabisz P, Wawak K, Wiśniewska A, Boratyn E, Nowak I, Horwacik I, Woźnicka O, Rokita H. (2018) GD2 ganglioside-binding antibody 14G2a and specific Aurora A kinase inhibitor MK-5108 induce autophagy in IMT-32 neuroblastoma cells. Apoptosis 23:492-511. doi: 10.1007/s10495-018-1472-9.
  6. Boratyn E, Nowak I, Durbas M, Horwacik I, Sawicka A, Rokita H. (2017) MCPIP1 exogenous overexpression inhibits pathways regulating MYCN oncoprotein stability in neuroblastoma. J. Cell. Biochem. 118:1741-1755. Doi.org/10.1002/jcb.25832. 
  7. Horwacik I, Rokita H. (2017) Modulation of interactions of neuroblastoma cell lines with extracellular matrix proteins affects their sensitivity to treatment with the anti-GD2 ganglioside antibody 14G2a. Int. J. Oncol. 50:1899-1914. Doi.org/10.3892/ijo.2017.3959.
  8. Durbas M, Horwacik I, Boratyn E, Rokita H. (2016) Downregulation of the PHLDA1 gene in IMR-32 neuroblastoma cells increases levels of Aurora A, TRKB and effects proteins involved in apoptosis and autophagy pathways. Int. J. Oncol. 49:823-837. Doi.org/10.3892/ijo.2016.3572.
  9. Boratyn E, Nowak I, Horwacik I, Durbas M, Mistarz A, Kukla M, Kaczówka P, Łastowska M, Jura J, Rokita H. (2016) Monocyte chemoattractant protein-1-induced protein 1 overexpression modulates transcriptome, including microRNA, in human neuroblastoma cells. J. Cell. Biochem. 117:694-707. doi: 10.1002/jcb.25354.
  10. Horwacik I, Golik P, Grudnik P, Koliński M, Zdzalik M, Rokita H, Dubin G. (2015) Structural basis of GD2 ganglioside and mimetic peptide recognition by 14G2a antibody. Mol. Cell. Proteomics. 14:2577-2590. doi: 10.1074/mcp.M115.052720.
  11. Horwacik I, Rokita H. (2015) Targeting of tumor-associated gangliosides with antibodies affects signaling pathways and leads to cell death including apoptosis. Apoptosis 20:679-688. doi: 10.1007/s10495-015-1103-7.
  12. Durbas M, Horwacik I, Boratyn E, Kamycka E, Rokita H. (2015) GD2 ganglioside specific antibody treatment down-regulates PI3K/Akt/mTOR signaling network in human neuroblastoma cell lines. Int. J. Oncol. 47:1143-1159. doi: 10.3892/ijo.2015.3070.

  • Studies on mechanisms of activity of monoclonal antibodies in cultured neuroblastoma cells
  • Studies on activity of chemotherapeutics on cultured neuroblastoma cell lines
  • Analysis of authophagy in human neuroblastoma cell lines
  • Analysis of types of cell death in neuroblastoma cells treated with a therapeutic antibody recognizing GD2 ganglioside and small molecular weight kinase inhibitors

  • Knowledge on biochemistry, molecular biology and English language